2013 Fiscal Year Final Research Report
Identification of substrate molecules on the host immune cells of bacterial proteases by using radioisotopes and mass spectrometry.
| Project/Area Number |
23510243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical genome science
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| Research Institution | Kumamoto University |
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Principal Investigator |
IRIE Atsushi 熊本大学, 生命科学研究部, 講師 (30250343)
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| Co-Investigator(Kenkyū-buntansha) |
IMAMURA Takahisa 熊本大学, 生命科学研究部, 准教授 (20176499)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 放射能標識 / 二次元電気泳動 / プロテオミクス / 免疫細胞 / 免疫逃避機構 |
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| Research Abstract |
Neutrophils from peripheral blood mononuclear cells of healthy volunteers were subjected to cell-surface radioactive-iodine labeling and to bacterial protease treatment. Neutrophils were so fragile and easily collapsed that it was difficult to separate proteins on the cell surface from those in the cytosol. Therefore, searching the conditions suitable for the radiolabeling and proteolysis for the neutrophils are underway. To recover the radioactive proteins from the gels, it was satisfactory to punch out the protein spots from dried gels sandwiched by cellophane sheets by using clean punches. It was necessary to prevent the exhaust air with trace amount of radioactivity from the vacuum pumps from diffusing in the laboratory to take care those people who do not use radioisotopes.
In this study, new effects of Staphylococcus aureus staphopains on fibrinogen and collagen were identified that account for the virulence activities of S aureus for impaired clotting and tissue destruction.
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[Journal Article] Identification of CDCAl long peptides bearing both CD4^+ and CD8^+ T-cell epitopes : CDCAl-specific CD4^+ T-cell immunity in cancer petients2014
Author(s)
Tomita Y, Yuno A, Tsukamoto H, Senju S, Yo shimura S, Osawa R, Kuroda Y, Hirayama M, Irie A, Hamada A, Jono H, Yoshida K, Tsunoda T, Kohrogi H, Yoshitake Y, Nakamura Y, Shinohara M, Nishimura Y
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Journal Title
Int J Cancer
Volume: 134
Pages: 352-366
DOI
Peer Reviewed
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[Journal Article] Identifcation of promiscuous KIF20A long peptides bearing both CD4^+ and CD8^+ T-cell epitopes:KIF20A-specifc CD4^+ T-cell immunity in patients with malignant tumor2014
Author(s)
Tomita Y, Akira Y, Tsukamoto H, Senju S, Kuroda Y, Hirayama M, Irie A, Kawahara K, Yatsuda J, Hamada A, Jono H, Yoshida K, Tsunoda T, Kohrogi H, Yoshitake Y, Nakamura Y, Shinohara M, Nishimura Y
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Journal Title
Ctin Cancer Res
Volume: 19
Pages: 4508-4520
DOI
Peer Reviewed
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[Journal Article] Establishment of HLA-DR4 transgenic mice for the identi_cation of CD4^+ T cell epitopes of tumor-associated antigens2013
Author(s)
Yatsuda J, Irie A, Harada K, Michibata Y, Tsukamoto H, Senju S, Tomita Y, Yuno A, Hirayama M, Abu Sayem M, Takeda N, Shibuya I, Sogo S, Fujiki F, Sugiyama H, Eto M, Nishimura Y
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Journal Title
J PLoS One
Volume: 8
Pages: e84908
DOI
Peer Reviewed
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[Journal Article] Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a-receptor (CD88)2013
Author(s)
Nitta H, Wada Y, Kawano Y, Murakami Y, Irie A, Taniguchi K, Kikuchi K, Yamada G,Suzuki K, Honda J, Wilson-Morifuji M, Araki N, Eto M, Baba H, Imamura T
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Journal Title
Ctin. Cancer. Res
Volume: 19
Pages: 2004-2013
DOI
Peer Reviewed
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[Journal Article] Selective inhibition of nuclear factor-κB by nuclear factor-κB essential modulator-binding domain peptide suppresses the metastasis of highly metastatic oral squamous cell carcinoma2012
Author(s)
Tanaka T, Nakayama H, Yoshitake Y, Irie A, Nagata M, Kawahara K, Takamune Y, Yoshida R, Nakagawa Y, Ogi H, Shinriki S, Ota K, Hiraki A, Ikebe T, Nishimura Y, Shinohara M
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Journal Title
Cancer Sci
Volume: 103
Pages: 455-463
DOI
Peer Reviewed
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[Journal Article] A novel tumor-associated antigen, cell division cycle 45-like can induce cytotoxic T-lymphocytes reactive to tumor cells2011
Author(s)
Tomita Y, Imai K, Senju S, Irie A, Inoue M, Hayashida Y, Shiraishi K, Mori T, Daigo Yp Tsunoda T, Ito T, Nomori H, Nakamura Y, Kohrogi H, Nishimura Y
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Journal Title
Cancer Sci
Volume: 102
Pages: 697-705
DOI
Peer Reviewed
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[Journal Article] Peptides derived from human insulin-like growth factor(IGF)-II mRNA binding protein 3 can induce human leukocyte antigen-A2-restricted cytotoxic T lymphocytes reactive to cancer cells2011
Author(s)
Tomita Y, Harao M, Senju S, Imai K, Hirata S, Irie A, Inoue M, Hayashida Y, Yoshimoto K, Shiraishi K, Mori T, Nomori H, Kohrogi H, Nishimura Y
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Journal Title
Caneer Sci
Volume: 102
Pages: 71-78
DOI
Peer Reviewed
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[Journal Article] Identification of HLA-A2-restricted CTL epitopes of a novel tumour-associated antigen, KIF20A, overexpressed in pancreatic cancer2011
Author(s)
Imai K, Hirata S, Irie A, Senju S, Ikuta Y, Yokomine K, Harao M, Inoue M, Tomita Y, Tsunoda T, Nakagawa H, Nakamura Y, Baba H, Nishimura Y
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Journal Title
Br J Cancer
Volume: 104
Pages: 300-307
DOI
Peer Reviewed
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[Presentation] Establishment of HLA-DR4(HLA-DRAIHLA-DRBI *04:05) transgenic mice2011
Author(s)
Irie A, Yatsuda J, Michibata Y, Harada K, Tomita Y, Kubo T, Imamura T, Takeda N, Shibuya I,Sogo S, Nishimura Y
Organizer
第40回日本免疫学会学術集会
Place of Presentation
幕張
Year and Date
20111127-29
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