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2013 Fiscal Year Final Research Report

Physiological function of paraspeckles: molecular mechanism of assembly and their nuclear distribution

Research Project

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Project/Area Number 23510247
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field System genome science
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

SASAKI Yasunori  独立行政法人産業技術総合研究所, バイオメディカル研究部門主任, 研究員 (30312242)

Project Period (FY) 2011 – 2013
Keywords核内構造体 / パラスペックル / ノンコーディングRNA / ゲノム
Research Abstract

Paraspeckle (PS) is a large RNA-protein complex, consists of several ten RNA binding proteins and NEAT1, a noncoding RNA indispensable for the nuclear body assembly. The current study focuses on the molecular mechanism and nuclear distribution of PS to ellucidate paraspeckles' physiological function.
We established a mouse cell constitutively expressing human NEAT1 RNA so that exogenous 'human PS' can be formed in addition to endogenous 'mouse PS'. In this heterologous system, 'human PS', a chimeric sturacutre of human RNA and mouse proteins was assembled independent of 'mouse PS'. Upon administration of a proteasome inhibitor, both humanPS and mouse PS enlarged independently. Furthermore, we observed that Neat1 loci (endogenous) and NEAT1 loci (exogenous), encoded separate chromosomes relocated vicinity, suggesting PS could be a driving force to recruit attached loci to close proximity.

  • Research Products

    (3 results)

All 2014 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (2 results)

  • [Journal Article] Alternative 3'-end processing of long noncoding RNA initiates construction of nuclear paraspeckles2012

    • Author(s)
      Naganuma T, Nakagawa S, Tanigawa A, Sasaki YF, Goshima N, Hirose T
    • Journal Title

      EMBO J

      Volume: 31巻 Pages: 4020-4034

    • Peer Reviewed
  • [Presentation] ノンコーディング RNA NEAT1 の Xenopus オーソログ2014

    • Author(s)
      佐々木保典、他9名
    • Organizer
      XCIJ 首都圏支部会
    • Place of Presentation
      横浜
    • Year and Date
      20140300
  • [Presentation] ノンコーディング RNA NEAT1のXenopus オーソログ2012

    • Author(s)
      佐々木保典、小川朝子、谷川明恵、小沼泰子、伊藤弓弦、廣瀬哲郎
    • Organizer
      日本 RNA 学会
    • Place of Presentation
      仙台
    • Year and Date
      20120700

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Published: 2015-07-16  

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