2013 Fiscal Year Final Research Report

Physiological function of paraspeckles: molecular mechanism of assembly and their nuclear distribution

Research Project

Project/Area Number	23510247
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	System genome science
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	SASAKI Yasunori 独立行政法人産業技術総合研究所, バイオメディカル研究部門主任, 研究員 (30312242)
Project Period (FY)	2011 – 2013
Keywords	核内構造体 / パラスペックル / ノンコーディングRNA / ゲノム
Research Abstract	Paraspeckle (PS) is a large RNA-protein complex, consists of several ten RNA binding proteins and NEAT1, a noncoding RNA indispensable for the nuclear body assembly. The current study focuses on the molecular mechanism and nuclear distribution of PS to ellucidate paraspeckles' physiological function. We established a mouse cell constitutively expressing human NEAT1 RNA so that exogenous 'human PS' can be formed in addition to endogenous 'mouse PS'. In this heterologous system, 'human PS', a chimeric sturacutre of human RNA and mouse proteins was assembled independent of 'mouse PS'. Upon administration of a proteasome inhibitor, both humanPS and mouse PS enlarged independently. Furthermore, we observed that Neat1 loci (endogenous) and NEAT1 loci (exogenous), encoded separate chromosomes relocated vicinity, suggesting PS could be a driving force to recruit attached loci to close proximity.