2014 Fiscal Year Final Research Report
Novel approaches for finding bioactive compounds with the assistance of the inverse analysis of food constituents
| Project/Area Number |
23510252
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Utsunomiya University |
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Principal Investigator |
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | 酵素阻害剤 / 配糖体 / ポリフェノール / 酸化生成物 |
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| Outline of Final Research Achievements |
Tyrosinase (polyphenol oxidase) is a copper-containing oxidoreductase, which is widely distributed in nature. Many monophenols are oxidized to corresponding o-quinones through this enzymatic reaction. Generated o-quinones are usually unstable and readily transformed into polymers including melanin. This polymer results in significant deposition of pigments on the skin. Accordingly, the study of tyrosinase inhibition is clearly important for the development of functional cosmetics as well as antioxidants and insecticides. On the other hand, reactive o-quinones are reduced to o-diphenols with the aid of reducing agents such as L-ascorbic acid and/or NADPH. Thus, unique o-diphenols could be generated from plant monophenols by tyrosinase-catalyzed oxidation coupled with in vivo reductants. To perform chemical and biological studies of inhibitors, substrates and products for tyrosinase, we have discovered a number of novel bioactive compounds.
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| Free Research Field |
天然物有機化学
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