2013 Fiscal Year Final Research Report
Molecular analysis of candidate proteins involved in ALA-PDT
| Project/Area Number |
23510256
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
TAKETANI Shigeru 京都工芸繊維大学, 工芸科学研究科, 教授 (20121949)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ALA / PDT / heme / protoporphyrin / mitochondria |
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| Research Abstract |
The ALA-induced accumulation of protoporphyrin and photodynamic therapy (PDT) have been used for the diagnosis of cancer. Several factors contribute to the high tumor specificity of ALA-induced accumulation of protoporphyrin as a detection marker for the photodiagnosis of tumors, and we gave evidences that protoporphyrin accumulates owing to the limited capacity for ferrochelatase reaction. Ferrochelatase consists of an iron-sulfur cluster and its expression in tumor cells was decreased due to the low expression of mitochondrial iron-chaperon frataxin unregulated by p53. Thus, mitochondrial dysfunctions, decreased utilization of iron in mitochondria and the recycling of heme-iron can be crucial factors for ALA-PDT. Additional factors including mitoferrin, siderflexin and ABCG2 are also involved in the accumulation of protoporphyrin. Tumor selectivity of ALA-PDT is due to the preferential uptake of ALA through neurotransmitter transporters from the blood stream.
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[Journal Article] 5-aminolevulinic acid combined with ferrous iron enhance the expression of heme oxygenase-12014
Author(s)
Y Nishio, M Fujino, M Zhao, T Ishii, M Ishizuka, H Ito, K Takahashi, F Abe, M Nakajima, T Tanaka, S Taketani, Y Nagahara, and X-K Li
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Journal Title
Intl Immunopharmacol
Volume: 19(2)
Pages: 300-307
DOI
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[Journal Article] Erythropoietin is involved in hemoprotein syntheses in developing human decidua2013
Author(s)
Shiota M, Yasuda Y, Shimaoka M, Tsuritani M, Koike E, Oiki M, Matsubara J, Taketani S, Murakami H, Yamasaki H, Okumoto K, Hoshiai H
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Journal Title
Congenit Anom (Kyoto)
Volume: 53(1)
Pages: 18-26
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[Journal Article] Eudesmane-type sesquiterpene lactones inhibit multiple steps in the NF-κB signaling pathway induced by inflammatory cytokines2012
Author(s)
Tamura R, Chen Y, Shinozaki M, Arao K, Wang L, Tang W, Hirano S, Ogura H, Mitsui T, Taketani S, Ando M, Kataoka T
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Journal Title
Bioorg. Med. Chem. Lett
Volume: 201222(1)
Pages: 207-211
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[Journal Article] Roles of porphyrin and iron metabolisms in the δ-aminolevulinic acid (ALA)-induced accumulation of protoporphyrin and photo-damage of tumor cells2011
Author(s)
Ohgari, Y. Miyata, Y., Miyagi, T., Gotoh, S., Ohta, T., Kataoka, T., Furuyama K., and Taketani, S
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Journal Title
Photochemistry & Photobiology
Volume: 87(5)
Pages: 1138-1145
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