2013 Fiscal Year Final Research Report
A convenient proteomic system for kinase substrate identification
| Project/Area Number |
23510261
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | Kitasato University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SATO Tatsuhiro 北里大学, 薬学部, 助教 (70547893)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
IIDA Naoyuki 北里大学, 薬学部, 講師 (10376618)
SHIBAGAKI Yoshio 北里大学, 薬学部, 講師 (90235565)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | プロテオミクス / キナーゼ / 二次元ゲル電気泳動 / リン酸化 / ERK / mTOR / p38 MAPキナーゼ |
|---|
| Research Abstract |
We established an in vitro dephosphorylation-phosphorylation system, by which we identified 7 novel p38 MAP kinase substrates. We also identified novel ERK and mTOR substrates as kinase-bound proteins. ERK phosphorylation of adducin attenuated adducin-actin filament interaction. Furthermore, we identified Filamin A as a novel mTOR substrate. mTOR phosphorylation of Filaimin A controls focal adhesions.
|
Research Products
(19 results)
-
-
-
-
-
[Journal Article] SIRPα interacts with nephrin at the podocyte slit diaphragm2012
Author(s)
Kajiho Y, Harita Y, Kurihara H, Horita S, Matsunaga A, Tsurumi H, Kanda S, Sugawara N, Miura K, Sekine T, Hattori S, Hattori M, Igarashi T
-
Journal Title
FEBS J.
Volume: 279
Pages: 3010-3021
DOI
-
-
-
[Journal Article] A novel CD3-specific antibody induces immunosuppression via impaired phosphorylation of LAT and PLC γ 1 following T-cell stimulation
Author(s)
Shiheido H, Aoyama T, Takahashi H, Hanaoka K, Abe T, Nishida E, Chen C, Koga O, Hikida M, Shibagaki Y, Morita A, Nikawa T, Hattori S, Watanabe T, Shimizu J
-
Journal Title
Eur J Immunol
Volume: in press
-
-
-
-
-
-
-
-
-
-
-
