2013 Fiscal Year Final Research Report
Development of versatile method for identification of the target protein using affinity resins with photo-labile linker
| Project/Area Number |
23510269
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Hyogo University of Health Sciences |
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Principal Investigator |
TANAKA AKITO 兵庫医療大学, 薬学部, 教授 (30454789)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 活性発現の分子機構 / アフィニティ樹脂 / 生理活性物質 / ターゲット探索 |
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| Research Abstract |
We have here develped an effective method for identification of bio-active compound such as drug, natural product, and so on, using affnity resins from lyates. In this method, we applied photo-labile linker to elute the target protein from affinity resins selectively after mixed with lysate from target organ. We used "AquaFirmus", our products, as solid material in this work because this is chemically stable and hydrophilic enough to reduce the non-specific protein absorption.
We first fixed target-known bioactive compounds such as FK506 or benzenesulfonamide on the resin through the photo-libile linker with hydrophilic spacers, and successfully eluted the target proteins by irradation on ice for 5 min. We now apply this novel method for identification of target-unknown compounds including developed compounds of pharmaceutical companies.
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