2013 Fiscal Year Final Research Report
Development of the reduction system of azo dyes by microbial enzymes using micro power as a driving force
| Project/Area Number |
23510275
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | Yamagata University |
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Principal Investigator |
KIJIMA Tatsuro 山形大学, 理工学研究科, 准教授 (50272084)
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| Co-Investigator(Renkei-kenkyūsha) |
OOI Toshihiko 北海道大学, 大学院工学研究科, 准教授 (40223713)
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| Project Period (FY) |
2011 – 2013
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| Keywords | フェロセン修飾補酵素 / NAD / アゾ還元酵素 / フェロセン / 補酵素再生系 |
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| Research Abstract |
Development of the coenzyme regeneration system for reduction of azo dyes by azoreductase on the electrode, NAD modificated by ferrocene moiety was synthesized and was active for four other NAD needed enzymes without inhibitions. Unfortunately, obtained ferrocene modified NAD was indicated low electrode activity. It was found that the coenzyme regeneration system for reduction of azo dyes by azoreductase with coupling to another reductase were promising for new biosensor of dramatic color change.
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[Journal Article] Flow-based Biosensing System for Glucose Fabricated by Using Track-etched Microporous Membrane Electrodes2013
Author(s)
Mizuguchi, H., Sakurai, J., Kinoshita, Y., Iiyama, M., Kijima, T., Tachibana, T., Nishina, T., Shida, J.
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Journal Title
Chemistry Letters
Volume: 42(10)
Pages: 1317-1319
Peer Reviewed
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