2013 Fiscal Year Final Research Report
Real-time detection of tyrosine phosphrylation by using lanthanide complexes
| Project/Area Number |
23510276
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | University of Tsukuba (2012-2013)
The University of Tokyo (2011) |
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Principal Investigator |
SUMAOKA JUN 筑波大学, 医学医療系, 講師 (10280934)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 希土類錯体 / リン酸化 / シグナル伝達 / イメージング |
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| Research Abstract |
We developed a binuclear Terbium(III) complexe which selectively responded to phosphotyrosine (pTyr) and emitted luminescence. With the use of the complex, phosphorylation of tyrosine and dephosphorylation of phosphotyrosine in peptides by protein tyrosine kinase(PTK) and by protein tyrosine phosphatase(PTP), respectively, were monitored in real-time. Furthermore, highly complicated systems, where a PTK and a PTP coexisted together, were analyzed by the present system. A straightforward and visual method to assess inhibitors on these enzymes was also developed.
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