Taxonomic evaluation of actinobacteria isolated from river basin and their preservation as genetic resources

2013 Fiscal Year Final Research Report

• Project/Area Number: 23510291
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Resource conservation science
• Research Institution: University of Yamanashi
• Principal Investigator: HAYAKAWA Masayuki 山梨大学, 医学工学総合研究部, 教授 (30126651)
• Co-Investigator(Kenkyū-buntansha): YAMAMURA Hideki 山梨大学, 医学工学総合研究部, 助教 (70516939)
• Project Period (FY): 2011 – 2013
• Keywords: 遺伝資源 / アクチノバクテリア / 生態学 / 分類学 / 微生物

Research Abstract

Actinobacteria are a group of Gram-positive and high GC content bacteria. The aim of the present study was to establish an effective isolation method for actinobacteria from river basin and evaluate their taxonomic diversity. By using a dilution plating method with 1/5 NBRC 802 medium containing 5% NaCl, a total of 194 actinobacteria were selectively isolated from the river and seashore sediments of Iriomote Island, Okinawa, Japan. According to the 16S rDNA homology search, they classified into 6 families, 10 genera and 23 species. Microbacterium thalassium was recognized as the most predominant species. Of 194 isolates, 48 strains were considered as belonging to new species. These results suggest that the river basin is a promising genetic resource for diverse and novel actinobacteria.

Research Products

• [Journal Article] Agromyces iriomotensis sp. nov. and Agromyces subtropicus sp. nov., isolated from soil (2014)
  • Author(s): Hamada, M., Shibata, C., Ishida, Y., Tamura, T., Yamamura, H., Hayakawa, M. & Suzuki, K.
  • Journal Title: Int J Syst Evol Microbioll. Volume: 64 Pages: 833-838
  • Peer Reviewed
• [Journal Article] Janibacter cremeus sp. nov., an actinobacterium isolated from sea sediment (2013)
  • Author(s): Hamada, M., Shibata, C., Tamura, T., Yamamura, H., Hayakawa, M. & Suzuki, K.
  • Journal Title: Int J Syst Evol Microbiol. Volume: 63 Pages: 3687-3690
  • Peer Reviewed
• [Journal Article] Paraoerskovia sediminicola sp. nov., an actinobacterium isolated from sea sediment, and emended description of the genus Paraoerskovia (2013)
  • Author(s): Hamada, M., Tamura, T., Shibata, C., Yamamura, H., Hayakawa, M., Schumann, P. & Suzuki, K.
  • Journal Title: Int J Syst Evol Microbiol. Volume: 63 Pages: 2637-2641
  • Peer Reviewed
• [Journal Article] Lysinimicrobium mangrovi gen. nov., sp. nov., an actinobacterium isolated from the rhizosphere of a mangrove (2012)
  • Author(s): Hamada, M., Tamura, T., Yamamura, H., Suzuki, K. & Hayakawa, M.
  • Journal Title: Int J Syst Evol Microbiol. Volume: 62 Pages: 1731-1735
  • Peer Reviewed
• [Presentation] マングローブ域土壌からのLysinimicrobium属放線菌の分離と分類学的研究 (2013)
  • Author(s): 浜田盛之、柴田千代、田村朋彦、小牧久幸、市川夏子、小口晃央、山村英樹、早川正幸、藤田信之、鈴木健一朗
  • Place of Presentation: メルパルク広島
  • Year and Date: 20130905-06
• [Presentation] 河川流域及び海洋環境からのアクチノバクテリアの分離 (2013)
  • Author(s): 石黒圭、山村英樹、中川洋史、浜田盛之、乙黒美彩、田村朋彦、早川正幸
  • Organizer: 日本農芸化学会関東支部2013年度支部大会
  • Place of Presentation: 慶応義塾大学日吉キャンパス
  • Year and Date: 2013-11-22
• [Presentation] 海洋堆積物からのDemequinaceae科放線菌の分離と分類学的研究 (2012)
  • Author(s): 浜田盛之、田村朋彦、山村英樹、早川正幸、鈴木健一朗
  • Organizer: 日本放線菌学会大会
  • Place of Presentation: 府中の森芸術劇場
  • Year and Date: 20120906-07
• [Remarks]
  • URL: http://www.ccn.yamanashi.ac.jp/~hyamamura/index.html