Approaches to the development of oral contraceptive vaccines for invasive raccoons (Procyon lotor)

2013 Fiscal Year Final Research Report

• Project/Area Number: 23510293
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Resource conservation science
• Research Institution: Gifu University
• Principal Investigator: ASANO MAKOTO 岐阜大学, 応用生物科学部, 准教授 (30377692)
• Co-Investigator(Kenkyū-buntansha): HANEDA Shingo 帯広畜産大学, 畜産学部, 助教 (40553441)
• Project Period (FY): 2011 – 2013
• Keywords: アライグマ / 避妊ワクチン / 特定外来生物 / 外来種 / 繁殖抑制 / 個体数管理

Research Abstract

In this study, we conducted the fundamental research about immunological fertility-control for introduced raccoons (Procyon lotor), particularly the zona pellucida 3 protein (ZP3) based oral vaccines. The full ZP3 gene including sperm combining site was sequenced. The synthetic peptides were designed on the basis of the amino acid sequence of PZ3 sperm combining site. The synthetic peptide vaccine was injected into four rabbits and the serum was obtained that contained the antibody to raccoon ZP3 sperm combining site. To verify the binding of the antibody to ZP, immune-histochemistry was performed using ovarian of raccoon, dog, cat, raccoon dog, Japanese badger, Japanese black bear and small Indian mongoose. The antibody obtained from one rabbit was able to bind raccoon ZP. However, the antibody-ZP binding was confirmed also in some other animals. The synthetic peptide vaccine used in this study had some issue as species-specificity, so further research should be needed.

Research Products

• [Journal Article] An attempt to induce antibody production for immunocontraception in the Hokkaido sika deer (Cervus nippon yesoensis) by immunization with a porcine zona pellucida synthetic peptide (2014)
  • Author(s): Kobayashi K., Asano M., Yanagawa Y., Haneda S. and Matsui M.
  • Journal Title: Mammal Study Volume: Vol39 Pages: 59-64
  • Peer Reviewed
• [Presentation] フイリマングース (Herpestes auropunctatus)の個体数抑制手法としての避妊ワクチン開発(2) (2013)
  • Author(s): 峰本隆博,淺野玄,森孝之,小林恒平,鈴木正嗣
  • Organizer: 第29回日本霊長類学会・日本哺乳類学会2013年度合同大会
  • Place of Presentation: 岡山
  • Year and Date: 20130000
• [Presentation] アライグマ(Procyon lotor)の経口避妊ワクチン開発の試み(1) (2013)
  • Author(s): 淺野玄,峰本隆博,小林恒平,鈴木正嗣
  • Organizer: 第29回日本霊長類学会・日本哺乳類学会2013年度合同大会
  • Place of Presentation: 岡山
  • Year and Date: 20130000
• [Presentation] Approaches to the development of fertility-control vaccines based on zona pellucida antigens for invasive small Indian mongoose (Herpestes auropunctatus) in Japan (2012)
  • Author(s): Asano, M., Mori, T., Kobayashi, K., Yamazaki, S., Minemoto, T. and Suzuki, M.
  • Organizer: 7th International Conference on Fertility Control in Wildlife
  • Place of Presentation: USA
  • Year and Date: 20120000
• [Presentation] フイリマングース (Herpestes auropunctatus)の個体数抑制手法としての避妊化ワクチン開発(1) (2012)
  • Author(s): 森孝之,淺野玄,小林恒平,峰本隆博,鈴木正嗣
  • Organizer: 日本哺乳類学会2012年度大会
  • Place of Presentation: 神奈川
  • Year and Date: 20120000