2013 Fiscal Year Final Research Report
Applications of Near-Infrared Raman Optical Activity to Biological Samples
| Project/Area Number |
23550019
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical chemistry
|
|---|
| Research Institution | Saga University |
|---|
Principal Investigator |
UNNO Masashi 佐賀大学, 工学(系)研究科(研究院), 准教授 (50255428)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
KAMO Naoki 松山大学, 薬学部, 教授 (10001976)
MASUDA Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 分子分光 / 振動分光 / 生物物理 / 光受容タンパク質 / 量子化学計算 |
|---|
| Research Abstract |
Many biological cofactors, such as light-absorbing chromophores in photoreceptors, are intrinsically planar molecules. A protein environment, however, causes structural distortions of the cofactor, and such structural changes can lead to a modulation of chemical properties of the cofactor to maximize its biological activity. We recently applied the near-infrared ROA to bacteriorhodopsin and photoactive yellow protein. These studies demonstrated that the near-infrared excitation allows us to measure the ROA spectra of a chromophore within a protein environment. Furthermore, calculations of the ROA spectra utilizing DFT provide detailed structural information, such as data about out-of-plane distortions of the chromophore. These findings extend the applicability of ROA to many biological cofactors.
|
Research Products
(31 results)
-
-
-
[Journal Article] Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analogue Revealed by Near-Infrared Raman Optical Activity2013
Author(s)
Kubota, K., Shingae, T., Foster, N. D., Kumauchi, M., Hoff, W. D. & Unno, M
-
Journal Title
J. Phys. Chem. Lett
Volume: 4
Pages: 3031-3038
Peer Reviewed
-
-
-
-
[Journal Article] Spectroscopic Evidence for the Formation of an N Intermediate during the Photocycle of Sensory Rhodopsin II (Phoborhodopsin) from Natronobacterium pharaonis2011
Author(s)
Tateishi, Y., Abe, T., Tamogami, J., Nakao, Y., Kikukawa, T., Kamo, N., & Unno, M
-
Journal Title
Biochemistry
Volume: 50
Pages: 2135-2143
Peer Reviewed
-
[Journal Article] Photochemistry of a Putative New Class of Sensory Rhodopsin (SRIII) coded by xop2 of Haloarcular marismortui2011
Author(s)
Nakao, Y., Kikukawa, T., Shimono1, K., Tamogami1, J., Kimitsuki, N., Nara, T., Unno, M., Ihara, K., & Kamo, N
-
Journal Title
J. Photochem. Photobiol. B
Volume: 102
Pages: 45-54
Peer Reviewed
-
-
-
-
-
-
[Presentation] Oral, Active site structure of photoactive yellow protein with a locked chromophore analogue revealed by near infrared Raman optical activity2014
Author(s)
Shingae, T., Kubota, K., Foster, N. D., Kumauchi, M., Hoff, W., Unno, M
Organizer
1st International Picobiology Institute Symposium, Picometer- to Micrometer-Level Structural Analyses for Studies on Functional Mechanisms of Proteins
Place of Presentation
Hyogo
Year and Date
20140107-08
-
-
-
[Presentation] Active Site Structure of Photoactive Yellow Protein with a Locked Chromophore Analog Revealed by Near Infrared Raman Optical Activity2013
Author(s)
Shingae, T., Kubota, K., Foster, N. D., Kumauchi, M., Hoff, W. D., Unno, M
Organizer
7th International Conference on Advanced Vibrational Spectroscopy
Place of Presentation
Kobe
Year and Date
20130825-30
-
-
-
-
-
-
-
-
-
-
-
-
-
-
