2013 Fiscal Year Final Research Report
High speed and precise evaluation of quality and physicochemical properties of pharmaceuticals by employing micro-analytical system such as CE
| Project/Area Number |
23550110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Yasuda Women's University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
NAGAMATSU Kumi 安田女子大学, 薬学部薬学科, 助手 (80614057)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 医薬品 / UHPLC / キャピラリー電気泳動 / エナンチオマー / 迅速分離 / 一斉分離 / 品質試験 |
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| Research Abstract |
We focused on capillary electrophoresis (CE) and ultra high-performance liquid chromatography (UHPLC), instead of the conventional HPLC, high speed and precise evaluation of quality and physicochemical properties of pharmaceuticals were investigated. Eenantiomers of diltiazem and its analogues etc. were successfully separated simultaneously by CE with dextrin as a chiral selector. Three enantiomers (e.g., six peaks) were separated within 15 min. By employing fluorescence detection in CE, high sensitive enantiomer separations of some DL-amino acids such as DL-Asp, DL-Ala were achieved. 4-Nitro-2,1,3-benzoxadiazole (e.g. NBD-F) was use as a derivatization reagent. In the separation by UHPLC, employing core-shell type columns, simultaneous separations of 6 cold medicines and 6 non-steroidal anti-inflammatory drugs (NSAIDs), 10 water soluble vitamins etc. were achieved within approximately 5 min with a relatively lower column pressure, compared with the conventional UHPLC columns.
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