2014 Fiscal Year Final Research Report
Elucidation of the mechanism of fibrinogen cryogelation: difference in the mechanism of fibrin gelation
| Project/Area Number |
23550237
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Polymer/Textile materials
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Kenji 群馬大学, 大学院理工学府, 教授 (10254567)
|
|---|
| Project Period (FY) |
2011-04-28 – 2015-03-31
|
| Keywords | フィブリノゲン / クライオゲル / フィブリン / 水晶振動子マイクロバランス / フィブリノゲン分解産物 |
|---|
| Outline of Final Research Achievements |
In this study, we have investigated the intermolecular interactions of fibrinogen molecules in the process of the cryogelation. The additive effects of urea and NaCl on the cryogelation were examined to elucidate the roles of hydrogen bonding and electrostatic interaction. The cryogelation were inhibited by the addition of urea and NaCl. The analysis of the experimental data suggested that the protofibrils were mainly formed by hydrogen bonding and the lateral aggregation of protofibrils was mainly regulated by electro static interaction. Furthermore, fibrinogen degradation products (FDPs) were made by plasmin to predict the interaction sites. Fragment X which is a FDP with loss of alpha C-chains could not form the cryogel, and therefore alpha C-chains are essential for the cryogelation.
|
| Free Research Field |
血液レオロジー
|
