2013 Fiscal Year Final Research Report
Preparation and biofunctional evaluation of PNA-PEG conjugates that can be applied to the genetic diseases caused by single nucleotide mutations.
| Project/Area Number |
23550243
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Tottori University |
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Principal Investigator |
SAKURAI Toshihiko 鳥取大学, 工学(系)研究科(研究院), 准教授 (10332868)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 人工核酸 / ペプチド核酸 / 遺伝子発現制御 / 1塩基認識 |
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| Research Abstract |
We synthesized the PNA-PEG conjugate and tried to regulate a gene expression using the cell free protein expression systems. As a result, the PNA-PEG conjugate inhibited the protein expression effectively and recognized the difference of the one base in vivo, compared with homo PNA oligomer. This was caused by the thermal stability at 30 degree, and this type of PNA-PEG conjugate was transported with macropinocytosis, but couldn't release to cytosol. with peptide signal (Q7-G-HSP), PNA-PEG conjugate was released to cytosol from mcropinosome, but it showed cytotoxicity that can not be ignored.
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