2013 Fiscal Year Final Research Report
Multistep Growth Mechanism of Calcium Phosphate in the Morphology-Controlled Biomineralization
| Project/Area Number |
23560925
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Reaction engineering/Process system
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KATO Katsuya 独立行政法人産業技術総合研究所, 先進製造プロセス研究部門, 研究グループ長 (70356781)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGUCHI Masahiro 名古屋工業大学, 工学研究科, 教授 (50357836)
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| Project Period (FY) |
2011 – 2013
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| Keywords | バイオミネラリゼーション / ペプチド / リン酸カルシウム / 自己組織化 / ヒドロキシアパタイト / アモルファス / リジン / グルタミン酸 |
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| Research Abstract |
We studied the effect of surface-functional-group position on precipitate morphology in the earliest stage of calcium phosphate biomineralization and determined the detailed mechanism of precipitation starting from nucleation to precipitate growth. The biomineralization template was a b-sheet peptide scaffold prepared by adsorption with carboxyl groups arranged at strict 0.7 nm; intervals. Phosphate was then introduced. Within 10 s, highly ordered embryos of calcium phosphate were formed and confined by a peptide nanofiber pattern. They repeatedly nucleated and dissolved, with the larger embryos absorbing the smaller ones in a clear demonstration of an Ostwald-ripening-like phenomenon, then aggregated in a line pattern, and finally formed highly ordered nanofibers of amorphous calcium phosphate. This multistep growth process constitutes the earliest stage of biomineralization.
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