2013 Fiscal Year Annual Research Report
| Project/Area Number |
23560940
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
VESTERGAAR d.C 北陸先端科学技術大学院大学, 大学院教育イニシアティブセンター, 特任准教授 (20582943)
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| Keywords | 免疫センサー / 流動性 / 毒性 / 神経保護 / アミロイド-β / 電気化学 / アルツハイマー病 / 生体模倣センサー |
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| Research Abstract |
We have shown that amyloid beta (Aβ) interacts with membranes in a species-dependent and membrane composition manner. They cause membrane transformation, breach and viscosity (Morita et al. 2012; Vestergaard et al. 2013; Morita et al. 2014)). These may contribute towards understanding the peptide’s toxicity mechanisms. Towards studying the influence of oxidized membranes on Alzheimer’s Aβ-induced toxicity, we studied how oxidation of cholesterol influences membrane dynamics (Vestergaard et al. 2011), demonstrating that temperature increase renders oxidized membranes more fluid. Similar findings have been found using biological cells (Phan et al., 2013; Phan et al., In Preparation). The findings are useful for clarifying the impact of cholesterol in Aβ-induced toxicity. Our preliminary studies on the role of oxidized cholesterol in Aβ-induced membrane dynamics show that oxidized membranes associate with and are destabilized more readily by A. Potential neuro-protective agents interact with membranes in a structure-dependent manner as does their ability to inhibit Aβ aggregation (Phan et al., Submitted; Samarat et al., In Preparation) . Unraveling the importance of these compounds, at a functional group level opens the key to tailored design of potential bioactive drug candidates. We have developed an immunosensor chip for Aβ. We have developed a gold nano-particle immunosensor chip for detecting amyloid beta. T(Lien et al., In Preparation). This system was presented at an Electrochemistry International conference (Vestergaard et al., Electrochem 2013, UK, 2013).
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[Journal Article] The effect of oxysterols on the interaction of Alzheimer's amyloid beta with model membranes2013
Author(s)
Phan, H.T.T., Hata, T., Morita, M., Yoda, T., Hamada, T., Vestergaard, M.C., Takagi, M.*
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Journal Title
Biochimica Biophysica Acta, Biomembranes
Volume: 1828
Pages: 2487-2495
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[Journal Article] Dynamic morphological changes induced by GM1 and protein interactions on the surface of cell-sized liposomes2013
Author(s)
Dhingra, S., Morita, M., Yoda, T., Vestergaard, M.C., Hamada, T., Takagi, M.*
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Journal Title
Materials
Volume: 6
Pages: 2522-2533
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