2013 Fiscal Year Final Research Report
Investigation of the protein-protein interactions in a bacterial polyhedral organelle and their application
| Project/Area Number |
23560943
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
MORI Ko-ichi 岡山大学, 大学院自然科学研究科, 助教 (50379715)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 生体触媒 / 細菌 / 細胞小器官 / ナノバイオ / バイオリアクター |
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| Research Abstract |
Considerable number of bacteria have proteinaceous polyhedral organelle in which metabolic reactions are carried to minimize the loss of volatile metabolic intermediates. In this study, we have developed expression system for polyhedral organelle genes, and analyzed their functions. We also found new interactions between some organelle enzymes, including diol dehydratase which was a key enzyme in the organelle metabolic pathway. These results suggest the importance of auto-assembly of the organelle enzymes in the formation process of polyhedral organelle.
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Research Products
(12 results)
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[Journal Article] Redesign of coenzyme B(12) dependent diol dehydratase to be resistant to the mechanism-based inactivation by glycerol and act on longer chain 1,2-diols2012
Author(s)
Yamanishi M., Kinoshita K., Fukuoka M., Saito T., Tanokuchi A., Ikeda Y., Obayashi H., Mori K., Shibata N., Tobimatsu T., Toraya T.
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Journal Title
FEBS Journal
Volume: 279(5)
Pages: 793-804
DOI
Peer Reviewed
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[Presentation] B12補酵素関与ジオールデヒドラターゼのグリセロールによる不活性化の機構と不活性化抵抗性酵素の再設計2012
Author(s)
虎谷哲夫, 山西守, 木下宏一郎, 福岡正喜, 齊藤拓也, 田野口亜耶, 池田祐輝, 大林弘和, 森光一, 柴田直樹, 飛松孝正
Organizer
ビタミンB研究委員会第427回研究協議会
Place of Presentation
京都市
Year and Date
2012-02-04
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