2013 Fiscal Year Final Research Report
Clean bioprocess design for immobilized enzyme reaction using a reduced pressure gas phase reactor
| Project/Area Number |
23560953
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kochi National College of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 減圧気相 / 酵素反応 / 固定化酵素 / 不斉合成 / バイオリアクター |
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| Research Abstract |
The chiral alcohol production from ketone catalyzed by immobilized alcohol dehydrogenase has been effectively proceeded in a gas phase bioreactor under reduced pressure, which was investigated for obtaining fundamental knowledge of clean bioprocess. First, a closed-type circular reactor was made to perform reduced pressure gas phase reaction. Next, after screening of the alcohol dehydrogenase species for effective proceed of reaction, the activity, stereoselectivity and reaction yield of the immobilized enzyme were measured as functions of the absolute pressure, the water and substrate concentrations in the reactor and the reaction temperature. Also, the immobilized enzyme activity was kinetically evaluated.
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