2013 Fiscal Year Final Research Report
Analysis of evolution and functions of plant clock system.
| Project/Area Number |
23570003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Nagoya University |
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Principal Investigator |
ONAI Kiyoshi 名古屋大学, 遺伝子実験施設, 研究員 (00402454)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 生物時計 / 概日リズム / 生物発光 / 生物発光リアルタイム測定法 / 原始紅藻 / 真核藻類 / 藍色細菌 / 高等植物 |
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| Research Abstract |
Circadian rhythms which are daily activities have been widely observed in many organisms from cyanobacteria to human. Properties of the rhythms are common among organisms but the clock genes which generate oscillation are not evolutionally conserved among plants. In this project, we aimed to be elucidate the evolution of plant clock genes and tried to isolate novel clock and clock-related genes from lower eukaryotic plant cells. We also analyzed the functions of clock proteins and their interactions with other proteins in cyanobacteria, and clock-controlled physiological phenomena in both prokaryotic and eukaryotic plants.
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[Journal Article] The promoter activities of sucrose phosphate synthase genes in rice, OsSPSJ and OsSPSJJ, are controlled by light and circadian clock, but not by sucrose2013
Author(s)
Yonekura M, Aoki N, Hirose T, Onai K, Ishiura M, Okamura M, Ohsugi R, Ohto C
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Journal Title
Frontiers Plant Sci.
Volume: 4(article)
Pages: 31
DOI
Peer Reviewed
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[Journal Article] The roles of the dimeric and tetrameric structures of the clock protein KaiB in the generation of circadian oscillations in cyanobacteria2012
Author(s)
Murakami R, Mutoh R, Iwase R, Furukawa Y, Imada K, Onai K, Morishita M, Yasui S, Ishii K, J. Valencia S., Uzumaki T, Namba K, and Ishiura M
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 29506-29515
DOI
Peer Reviewed
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[Journal Article] Aquaporin AqpZ is involved in cell volume regulation and sensitivity to osmotic stress in Synechocystis sp. strain PCC 68032012
Author(s)
Akai M, Onai K, Morishita M, Mino H, Shijuku T, Maruyama H, Arai F, Itoh S, Hazama A, Checchetto V, Szabo I, Yukutake Y, Suematsu M, Yasui M,. Ishiura M, Uozumi N
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Journal Title
J. Bacteriol.
Volume: 194
Pages: 6828-6836
DOI
Peer Reviewed
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[Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp.PCC 68032011
Author(s)
Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M, Uozumi N
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 25224-25235
DOI
Peer Reviewed
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[Remarks] プレス発表及び新聞記事・WEB記事:松尾拓哉、石浦正寛、小内清、丹羽由実(科学技術振興機構、名古屋大学共同発表);「緑藻の体内時計～時刻合わせの分子メカニズムを解明～」;発表日:2013年7月25日;場所:国立大学法人名古屋大学本部4号館第8会議室
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[Remarks] 上記プレス発表に関する新聞記事とWEB記事 中日新聞(2013年7.月30日朝刊) 科学新聞(2013年8.月2日、1面) 日経産業新聞(2013年8月7日) 時事ドットコム(2013年7月30日)
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[Remarks] 植物の小型種子を固体培地に自動で播種する「小型自動種まき機」を中立電機株式会社と共同開発して実用化した(2013年5.月に中立電機株式会社および椿本興業株式会社より商品名「アクアシード」として販売を開始した)。
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[Remarks] 温調とCO_2ガス制御対応の「高感度生物発光測定装置」を中立電機株式会社と共同開発して実用化した(2013年12月に中立電機株式会社、コスモ・バイオ株式会社、椿本興業株式会社から商品名「高感度生物発光測定装置CL24AおよびCL96A」として販売を開始した)。
