Dynamics of organelles and its regulation by membrane trafficking in Drosophila male meiotic division

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570004
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: INOUE Yoshihiro 京都工芸繊維大学, 昆虫バイオメディカル教育研究センター, 准教授 (90201938)
• Co-Investigator(Kenkyū-buntansha): YAMAGUCHI Masamitsu 京都工芸繊維大学, 工芸科学研究科, 教授 (00182460)
• Project Period (FY): 2011 – 2013
• Keywords: ショウジョウバエ / 減数分裂 / オルガネラ / 細胞周期

Research Abstract

Drosophila primary spermatocytes undergoing meiotic division I have advantages that one can easily observe intracellular structures and can examine regulatory mechanism of later stages of cell divisions. We established multi-color imaging system that makes us possible to perform a time-lapse observation of cell organelles as a progression of meiotic divisions. We carried out depletion experiments of 1030 genes that are possibly involved in membrane trafficking. Consequently, we identified COPI and COPII components required for intracellular vesicle transport as essential factors of cytokinesis in male meiosis. The COPI is required for initiation of cytokinesis by mediating transport of cleavage furrow proteins or lipids toward cleavage sites. On the other hand, the COPII is indispensable for a progression of cytokinesis through maintenance of connection between contractile ring and plasma membrane. However, larval neuroblasts neither need both coatomer complexes.

Research Products

• [Journal Article] CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis (2014)
  • Author(s): Kitazawa, D., Matsuo, T., Kaizuka, K., Miyauchi, C., Hayashi, D. and Inoue, Y. H. Orbit
  • Journal Title: PLoS ONE Volume: 9(5) Pages: e93669
  • DOI: 10.1371/journal.pone.0093669
  • Peer Reviewed
• [Journal Article] Drosophila MCM10 is required for DNA replication and differentiation in the compound eye (2014)
  • Author(s): Vo, N., Taga, A., Inaba, Y., Hideki, Y., Cotterill, S. and Yamaguchi, M.
  • Journal Title: PLoS ONE Volume: 9(PMID:24686397) Pages: e93450
  • DOI: 10.1371/journal.pone.0093450
  • Peer Reviewed
• [Journal Article] Identification of ter94, Drosophila VCP, as a strong modulator of motor neuron degeneration induced by knockdown of Caz, Drosophila FUS (2014)
  • Author(s): Azuma, Y., Tokuda, T., Shimamura M., Kyotani, A., Sasayama H., Yoshida, T., Mizuta, I., Mizuno, T., Nakagawa, M., Fujikake, N., Ueyama, M., Nagai, Y. and Yamaguchi, M.
  • Journal Title: Hum. Mol. Genet. Volume: (In press)
  • Peer Reviewed
• [Journal Article] CLASP is required for germline cyst formation through its developmental control of fusomes and ring canals in Drosophila males. : An essential gene for male germline cyst formation (2013)
  • Author(s): Miyauchi, C., Kitazawa, D., Ando, I. and Inoue, Y. H. Orbit
  • Journal Title: PLoS One Volume: 8 Pages: e58220
  • DOI: 10.1371/journal.pone.0058
  • Peer Reviewed
• [Journal Article] Elimination of Y chromosome-bearing spermatids during spermiogenesis in an autosomal sex-ratio mutant of Drosophila simulans (2013)
  • Author(s): Yasuno Y., Inoue Y. H. and M. Yamamoto
  • Journal Title: Gene. Genet. Syst. Volume: 88 Pages: 113-26
  • URL: https://www.jstage.jst.go.jp/article/ggs/88/2/88_113/_pdf
  • Peer Reviewed
• [Journal Article] The Drosophila DOCK family protein Sponge is involved in differentiation of R7 photoreceptor cells (2013)
  • Author(s): Eguchi, K., Yoshioka, Y., Yoshida, H., Morishita, K., Miyata, S., Hiai, H. and Yamaguchi, M.
  • Journal Title: Exp. Cell Res. Volume: 315 Pages: 2179-2195
  • Peer Reviewed
• [Journal Article] Distribution and morphological changes of the Golgi apparatus during Drosophila spermatogenesis (2013)
  • Author(s): Yasuno, Y., Inoue, Y. H. and Yamamoto, M.
  • Journal Title: Dev.Growth.Differ. Volume: 55 Pages: 635-647
  • DOI: 10.1111/dgd.12070
• [Journal Article] COPI-mediated membrane trafficking is required for cytokinesis in Drosophila male meiotic divisions (2012)
  • Author(s): Kitazawa, D., Yamaguchi, M., Mori, H. Inoue, Y. H.
  • Journal Title: J. Cell Sci. Volume: 125 Pages: 3649-3660
  • DOI: 10.1242/jcs.103317
  • Peer Reviewed
• [Journal Article] XNP/dATRX interacts with DREF in the chromatin to regulate gene expression (2012)
  • Author(s): Valadez-Graham, V., Yoshioka, Y., Velazquez, O., Kawamori, A. Vazquez, M., Neumann, A., Yamaguchi, M. and Zurita, M.
  • Journal Title: Nucleic Acids Res. Volume: 40 Pages: 1460-1474
  • DOI: 10.1093/nar/gkr865
  • Peer Reviewed
• [Journal Article] Polycomb repressive complex 2-dependent and –independent functions of Jarid2 in transcriptional regulation in Drosophila (2012)
  • Author(s): Herz, H.-M., Mohan, M., Garrett, A.-S., Miller, C., Casto, D., Zhang, Y., Seidel, C., Haug, J. S., Florens, L., Washburn, M. P., Yamaguchi, M., Shiekhattar, R. and Shilatifard, A.
  • Journal Title: Mol. Cell. Biol. Volume: 32 Pages: 1683-1693
  • DOI: 10.1128/MCB.06503-11
  • Peer Reviewed
• [Journal Article] MLF is a conserved regulator of RUNX transcription factor activity involved in hematopoiesis (2012)
  • Author(s): Bras, S., Martin-Lanneree, S., Gobert, V., Auge, B., Breig, O., Sanial, M., Yamaguchi, M., Haenlin, M., Plessis, A. and Waltzer, L.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Pages: 4986-4991
  • DOI: 10.1073/pnas.1117317109
  • Peer Reviewed
• [Journal Article] Roles of histone H3K9 methyltransferases during Drosophila spermatogenesis (2012)
  • Author(s): Ushijima Y., Inoue, Y. H., Konishi, T., Kitazawa, D., Yoshida, H., Shimaji, K., Kimura, H. and Yamaguchi, M.
  • Journal Title: Chromosome Res. Volume: 20 Pages: 319-331
  • DOI: 10.1007/s10577-012-9276-1
  • Peer Reviewed
• [Journal Article] Knockdown of the Drosophila Fused in Sarcoma (FUS) homologue causes deficient locomotive behavior and shortening of motoneuron terminal branches (2012)
  • Author(s): Sasayama, H., Shimamura, M., Tokuda, T., Azuma, Y., Yoshida, T., Mizuno, T., Nakagawa, M., Fujikake, N., Nagai, Y. and Yamaguchi, M.
  • Journal Title: PLos One Volume: 7 Pages: e39483
  • DOI: 10.1371/journal.pone.0039483
  • Peer Reviewed
• [Journal Article] Essential role of Duox in stabilization of Drosophila wing (2011)
  • Author(s): N. T. T. Anh, M. Nishitani, S. Harada, M. Yamaguchi, and K. Kamei
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 33244-33251
  • DOI: 10.1074/jbc.M111.263178
  • Peer Reviewed
• [Journal Article] S. DNA polymerase alpha interacts with PrSet7 and mediates H4K20 monomethylation in Drosophila
  • Author(s): Sahashi, R., Crevel G., Pasko, J., Suyari, O., Nagai, R., Saura, M. M., Yamaguchi, M. and Cotterill
  • Journal Title: J. Cell Science Volume: (In press)
  • Peer Reviewed
• [Presentation] Developmental control of Drosophila spermatogenesis by Clasp (2014)
  • Author(s): Inoue, Y. H.
  • Organizer: Orbit International symposium of IBRC in KIT
  • Place of Presentation: Kyoto
  • Year and Date: 2014-03-19
  • Invited
• [Presentation] Targeted induction of ER stress in Insulin-Producing Cells results in diabetes-like phenotype,inhibition of cell growth and dwarfism in Drosophila (2013)
  • Author(s): Hinami, Y. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 20131203-06
• [Presentation] Sir2 can induce 4EBP and Catalase gene expression through Foxo activation in Drosophila (2013)
  • Author(s): Nakahara, Y. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 20131203-06
• [Presentation] Identification of target genes of MXC protein, a tumor suppressor gene produ ct, and its transctiptional regulation in Drosophila (2013)
  • Author(s): Awane, R. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 20131203-06
• [Presentation] Proper mitochondria dynamics of mitochondria is required for meiotic cell cycle progression in Drosophila spermatogenesis (2013)
  • Author(s): Matsuo, T. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 20131203-06
• [Presentation] COPII components are required for later stage of cytokinesis in Drosophila meiotic divisions (2013)
  • Author(s): Kaizuka, K. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 20131203-06
• [Presentation] Orbit/CLASP is an essential factor for elongation that mature centrioles in Drosophila primary spermatocytes (2013)
  • Author(s): Asano, Y. and Inoue, Y. H.
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウニンク愛知
  • Year and Date: 20130619-21
• [Presentation] 被覆小胞COPIIはショウジョウバエ雄減数分裂における細胞質分裂に必要である (2013)
  • Author(s): 貝塚加奈, 井上喜博
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: 名古屋、ウニンク愛知
  • Year and Date: 20130619-21
• [Presentation] 7SKsnRNP components such as LARP7 and 7SKsnRNP are dispensable for viability and fertility in Drosophila (2013)
  • Author(s): Okazaki, R. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 2013-12-03
• [Presentation] A role of mxc gene in larval lymph gland in Drosophila hematopoiesis (2013)
  • Author(s): Ozeki, T. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸、国際会議場
  • Year and Date: 2013-12-03
• [Presentation] Accumulation of ROS promotes muscle senescence in Drosophila adults and also influences muscle development (2013)
  • Author(s): Hirai, J. and Inoue, Y. H.
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市
  • Year and Date: 2013-12-03
• [Presentation] Nuclear envelope proteins, Lamins and nuclear pore complex are required for cytokinesis in Drosophila male meiosis (2013)
  • Author(s): Hayashi, D. and Inoue, Y. H.
  • Organizer: EMBO Work Shop 2013 Drosophila Cell division cycle
  • Place of Presentation: Darlington
  • Year and Date: 2013-09-12
  • Invited
• [Remarks]
  • URL: https://sites.google.com/site/ibmrcdrosophila/
• [Patent(Industrial Property Rights)] 抗老化物質のスクリーニング方法 (2014)
  • Inventor(s): 井上喜博
  • Industrial Property Rights Holder: 京都工芸繊維大学
  • Industrial Property Number: 特願2014-063045
  • Filing Date: 2014-03-26
  • Overseas