2013 Fiscal Year Final Research Report
Regulation of the senescence responsive genes by nucleosomal change
| Project/Area Number |
23570008
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Genetics/Genome dynamics
|
|---|
| Research Institution | Yokohama City University |
|---|
Principal Investigator |
AYUSAWA dai 横浜市立大学, その他の研究科, 教授 (00142109)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | 細胞老化 / ヌクレオソーム / 5-ブロモデオキシウリジン |
|---|
| Research Abstract |
In this study, we verified whether 5-bromodeoxyuridine can change nucleosomal structure in the promoter region of the PAI-1 gene quickly responsive to 5-bromodeoxyuridine in human HeLa cells. We developed "the primer extension method" to precisely analyze the positioning of nucleosomes on DNA. Digestion of the chromatin DNA with micrococcal nuclease followed by linker ligation and PCR with specific primers revealed specific bands that are thought to be derived from digestion with the nuclease. With efforts to eliminate non-specific bands, we came to a situation where we can precisely detect a change in the nucleosommal structure.
|
Research Products
(3 results)
