2013 Fiscal Year Final Research Report
Study on the interaction of epigenetic factors and regulation of gene expression in higher plant embryogenesis
Project/Area Number |
23570045
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | University of Tsukuba |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Akira 筑波大学, 生命環境系, 准教授 (00400648)
|
Project Period (FY) |
2011 – 2013
|
Keywords | 胚発生 / エピジェネティクス / 成長相転換 |
Research Abstract |
In order to evaluate whether the inhibition of growth after germination induced by histone deacethylase (HDAC) inhibitors was a universal phenomenon in higher plants, Trichostatin A treatment was performed to three Arabidopsis related species. As a result, transition to the vegetative phase was inhibited in all, and it was suggested that HDAC is involved in this transition universally. On the other hand, expression of C-LEC1 gene and DNA methylation level in upstream region of C-LEC1 were observed peaks in early timing of embryo induction in carrot. The transiently increasing of DNA methylation was derived from CxG and CHH contexts that might be controlled by de novo type methylase.
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