2013 Fiscal Year Final Research Report
Functional analysis of hisitidine kinases by expression of chimeric sensors
| Project/Area Number |
23570046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
SUZUKI Iwane 筑波大学, 生命環境系, 教授 (10290909)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 二成分制御系 / ヒスチジンキナーゼ / キメラタンパク質 / 遺伝子発現 / 環境応答 |
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| Research Abstract |
Most of bacteria utilize hisitdine kinases as signal sensors for perception of changes in environmental conditions. Generally histidine kinase consists of signal-input domain and kinase domain. The formers are diverse and the laters are well-conserved.
We expressed chimeric sensors which contain signal-input domain from uncharacterised histidinekinase and the kinase domain from SphS, a phosphate-deficient sensor from the cyanobacterium Synechocystis sp. PCC 6803. We identified that a chimeric sensor of Hik2, one of the essential hisitidine kinase, specifically responded to salt stress by sencing Chloride ion concentration. It was the first finding that the salt sensor perceives chloride ion.
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