Structure and function of arabinogalactan-proteins in cell walls of higher plants

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570048
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: TSUMURAYA Yoichi 埼玉大学, 理工学研究科, 教授 (10142233)
• Project Period (FY): 2011 – 2013
• Keywords: 植物細胞壁 / 細胞壁多糖 / アラビノガラクタン-プロテイン / ガラクタナーゼ / エノキタケ / ダイコン

Research Abstract

Plant cell walls consist mainly of cellulose and other polysaccharides, such as pectins and hemicelluloses as well as arabinogalactan-proteins (AGPs), a family of proteoglycans/glycoproteins. In this study, our laboratory has focused to explore a new galactanase hydrolyzing the carbohydrate moieties of AGPs in various microbial sources. We found an endo-beta-1,3-galactanase from winter mushroom and demonstrated that the enzyme is highly useful to elucidate the structure of AGPs. A model structure of the carbohydrate moieties of Arabidopsis leaf AGP has been proposed by application of several different kinds of AGP-degrading enzymes. Similar approach allowed to determine the structure of radish root AGP. We also appeared that the enzymatically degraded AGP fragments possess physiological function (elicitor activity) producing antimicrobial substances when administered to soybean cotyledons.

Research Products

• [Journal Article] Enzymatic fragmentation of carbohydrate moieties of radish arabinogalactan-protein and elucidation of the structures (2014)
  • Author(s): R. Shimoda, K. Okabe, T. Kotake, K. Matsuoka, T. Koyama, T. Tryfona, H.-C. Liang, P. Dupree, Y. Tsumuraya
  • Journal Title: Biosci. Biotech. Biochem. Volume: (in press)
• [Journal Article] Aβ-glucuronosyltransferase from Arabidopsis thaliana involved in biosynthesis of type II arabinogalactan has a role in cell elongation during seedling growth (2013)
  • Author(s): Knoch, A. Dilokpimol, T. Tryfona, C.P. Poulsen, G. Xiong, J. Harholt, B.L. Petersen1, P. Ulvskov1, M.Z. Hadi, T. Kotake, Y. Tsumuraya, M. Pauly, P. Dupree, N. Geshi
  • Journal Title: Plant J. Volume: 76 Pages: 1016-1029
• [Journal Article] Biosynthesis of the carbohydrate moieties of arabinogalactan proteins by membrane-boundβ-glucuronosyltransferases from radish primary roots (2013)
  • Author(s): M. Endo, T. Kotake, Y. Watanabe, K. Kimura, and Y. Tsumuraya
  • Journal Title: Planta Volume: 238 Pages: 1157-1169
• [Journal Article] β-Galactosyl Yariv reagent binds to theβ-1,3-galactan of arabinogalactan proteins (2013)
  • Author(s): K. Kitazawa, T. Tryfona, Y. Yoshimi, Y. Hayashi, S. Kawauchi, L. Antonov, H. Tanaka, T. Takahashi, S. Kaneko, P. Dupree, Y. Tsumuraya, and T. Kotake
  • Journal Title: Plant Physiol. Volume: 161 Pages: 1117-1126
• [Journal Article] Structural characterisation of Arabidopsis leaf arabinogalactan polysaccharides (2012)
  • Author(s): T. Tryfona, H.-C. Liang, T. Kotake, Y. Tsumuraya, E. Stephens, and P. Dupree
  • Journal Title: Plant Physiol. Volume: 160 Pages: 653-666
• [Journal Article] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes (2011)
  • Author(s): T. Kotake, N. Hirata, Y. Degi, M. Ishiguro, K. Kitazawa, R. Takata, H. Ichinose, S. Kaneko, K. Igarashi, M. Samejima, and Y. Tsumuraya
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 27848-27854
• [Presentation] カラマツアラビノガラクタンにおけるβ-ヤリブ試薬の特異的相互作用部位 (2014)
  • Author(s): 佐藤一樹, 北澤仁成, 吉見圭永, 小竹敬久, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
  • Year and Date: 20140318-20
• [Presentation] エノキタケ由来のコアタンパク質分解酵素の精製 (2014)
  • Author(s): 吉見圭永, 菅原優美, 金子哲, 五十嵐圭日子, 小竹敬久, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 富山
  • Year and Date: 20140318-20
• [Presentation] Specific interaction ofβ-galactosyl Yariv reagent withβ-1,3-galactan (2013)
  • Author(s): T. Kotake, K. Kitazawa, T. Tryfona, Y. Yoshimi, S. Kaneko, P. Dupree, and Y. Tsumuraya
  • Organizer: The XI Cell Wall Meeting
  • Place of Presentation: Nantes, France
  • Year and Date: 20130707-12
• [Presentation] β-ヤリブ試薬とAGPのβ-1,3-ガラクタンの特異的相互作用 (2013)
  • Author(s): 吉見圭永, 小竹敬久, 北澤仁成, T. Tryfona, 金子哲, P. Dupree, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 岡山
  • Year and Date: 20130321-23
• [Presentation] 細胞壁グルコマンナン合成におけるKONJAC1,2の役割 (2012)
  • Author(s): 小竹敬久, J. Mortimer, 田島憲明, 宮崎祐一, P. Dupree, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] Structure of the sugar chains in radish arabinogalactan-protein (2011)
  • Author(s): R. Shimoda, K. Okabe, T. Kotake, K. Matsuoka, T. Koyama, H.-C. Liang, P. Dupree, Y. Tsumuraya
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: Awaji, JAPAN
  • Year and Date: 20111002-06
• [Presentation] Endo-β-1,3-galactanase from winter mushroom Flammulina velutipes and its action on arabinogalactan-protein (2011)
  • Author(s): T. Kotake, N. Hirata, Y. Degi, M. Ishiguro, K. Kitazawa, R. Takata, H. Ichinose, S. Kaneko, K. Igarashi, M. Samejima, and Y. Tsumuraya
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: Awaji, JAPAN
  • Year and Date: 20111002-06
• [Presentation] アラビノガラクタン-プロテイン糖鎖を分解するエンド-ガラクタナーゼ (2011)
  • Author(s): 平田尚弘, 出木雄太, 北澤仁成, 高田遼平, 小竹敬久, 一ノ瀬仁美, 金子哲, 石黒真希, 五十嵐今日子, 鮫島正弘, 円谷陽一
  • Organizer: 日本糖質学会
  • Place of Presentation: 長岡
  • Year and Date: 20110711-13
• [Book] 植物細胞壁多糖 (2013)
  • Author(s): 円谷陽一
  • Total Pages: 総ページ349(5章分担執筆)
  • Publisher: 講談社(西谷和彦、梅澤俊明編集)
• [Remarks] 生体物質研究室
  • URL: http://www.molbiol.saitama-u.ac.jp/bussitsu/index_j.html
• [Remarks] 埼玉大学 理学部 分子生物学科
  • URL: http://www.molbiol.saitama-u.ac.jp/index.html