2013 Fiscal Year Final Research Report
The study on lipid modification of extrinsic proteins and thermotorelance of photosynthesis
| Project/Area Number |
23570049
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Hosei University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 光化学系２ / 光合成 / 高温ストレス / シアノバクテリア / 脂質 |
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| Research Abstract |
Mechanism of thermotorelance of cyanobacterial photosystem II complex (PSII) has been investigated using Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 focusing on the role of lipids and extrinsic proteins in stabilization of PSII. It was found that at least one phosphatidylglycerol molecule associated with the reducing side of PSII is involved in the stabilization of PSII through affecting not only the reducing-side but also the oxidizing-side of PSII, namely, functional binding of extrinsic proteins to oxygen-evolving complex. We made an Anabaena mutant in which a histidine-tag was attached to one of subunits of PSII to allow the isolation of highly purified PSII complex rapidly from the cells.
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[Journal Article] Cyanobacterial monogalactosyldiacylglycerol-synthesi s pathway is involved in normal unsaturation of galactolipids and low-temperature adaptation of Synechocystis sp. PCC 68032014
Author(s)
Yuzawa, Y., Shimojima, Sato R., Mizusawa N., Yamamichi K., Suzuki, M., Iwai, M., Hori, K., Wada, H., Masuda, S., Ohta, H.
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Journal Title
Biochim. Biophys. Acta
Volume: 1841
Pages: 475-483
Peer Reviewed
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[Journal Article] Essential role of digalactosyldiacylglycerol for photosynthetic growth in Synechocystis sp. PCC 6803 under high-temperature stress2013
Author(s)
Mizusawa, N., Sakata, S., Sakurai, I., Kubota, H., Sato N., Wada, H.
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Journal Title
In Photosynthesis Research for Food, Fuel and Future (Kuang, T., Lu, C., Zhang, L., eds., Springer)
Pages: 620-624
Peer Reviewed
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[Journal Article] Quality control of photosystem II; Lipid peroxidation accelerates photoinhibition under excessive illumination2012
Author(s)
Chan, T., Shimizu, Y., Pospíšil, P., Nijo, N, Fujiwara A., Taninaka, Y. Imai, A. Morita, N., Yoshioka-Nishimura M., Izumi Y., Yamamoto,Y., Kobayashi, H., Mizusawa, N., Wada, H., Yamamoto, Y.
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Journal Title
Plos one
Volume: 7
Pages: e52100
DOI
Peer Reviewed
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[Presentation] Indispensable function of lipids in photosynthesis2012
Author(s)
Wada, H., Sakurai, I., Mizusawa, N., Ujihara, T., Tanoue, R., Endo, K., Kobayashi, K.
Organizer
Okayama university international symposium "Structure and Dynamics of Photosynthetic Systems"
Place of Presentation
岡山大学、岡山県
Year and Date
20121022-23
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[Presentation] Complementation analysis of cyanobacterial monoglucosyldiacylglycerol synthase by higher plant monogalactosyldiacylglycerol synthase in Synechocystis sp. PCC 68032011
Author(s)
Yuzawa, Y., Shimojima, M., Mizusawa, N., Sato, R., Iwai, M., Wada, H., Masuda, S., Ohta, H.
Organizer
The 4th Asian symposium on plant lipids
Place of Presentation
香港大学、香港
Year and Date
20111202-04
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