Analysis of nitrogen fixation system using host plant symbiotic mutants

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570052
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Aichi University of Education
• Principal Investigator: NORIO Suganuma 愛知教育大学, 教育学部, 教授 (40179114)
• Project Period (FY): 2011 – 2013
• Keywords: 共生 / 根粒 / 窒素固定 / 変異体 / ミヤコグサ

Research Abstract

Leguminous plants fix atmospheric nitrogen gas in symbiotic association with rhizobia. In order to establish a molecular basis for legume and rhizobial symbiotic nitrogen fixation, we studied Fix- mutants of the model legume, Lotus japonicus, and tried to identify the responsible genes and reveal the function of those genes. One of Fix- mutants was shown to be defective in a member of Soluble N-Ethylmaleimide Sensitive Factor Attachment Protein Receptor (SNARE) proteins involved in vesicle trafficking, which is expressed ubiquitously in shoot, roots, and nodules and the transcripts are detected in the vascular tissues.

Research Products

• [Journal Article] 共生菌の遺伝子欠落を補う宿主植物 (2013)
  • Author(s): 箱山雅生, 河内宏, 菅沼教生
  • Journal Title: 生物の科学遺伝 Volume: 67 Pages: 441-446
• [Journal Article] The SNARE protein SYP71 expressed in vascular tissues is involved in symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Hakoyama, T., Oi, R., Suga, E., Adachi, Y., Kobayashi, M., Akai, R., Sato, S., Fukai, E., Tabata, S., Shibata, S., Wu, G.J., Hase, Y., Tanaka, A., Kawaguchi, M., Kouchi, H., Umehara, Y., and Suganuma, N.
  • Journal Title: Plant Physiol Volume: 160 Pages: 897-905
  • DOI: 10.1104/pp.112.200782
  • Peer Reviewed
• [Journal Article] The integral membrane protein SEN1 is required for symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Hakoyama, T., Niimi, K., Yamamoto, T., Isobe, S., Sato, S., Nakamura, Y., Tabata, S., Kumagai, H., Umehara, Y., Brossuleit, K., Petersen, T.R., Sandal, N., Stougaard, J., Udvardi, M.K., Tamaoki, M., Kawaguchi, M., Kouchi, H., and Suganuma, N.
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 225-236
  • DOI: 10.1093/pcp/pcr167
  • Peer Reviewed
• [Presentation] Analysis of a host factor required for efficient symbiotic nitrogen fixation in model and crop plants–Functional analysis of Lotus japonicus SEN1 protein- (2013)
  • Author(s): Hakoyama, T., Suganuma, N., Kouchi, H., Hayashi, M. and Fujiwara, T.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 20131014-18
• [Presentation] 窒素固定能の発現に必須なミヤコグサSEN1タンパク質の機能解析 (2013)
  • Author(s): 箱山雅生, 菅沼教生, 河内宏, 林誠, 藤原徹
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学
  • Year and Date: 20130321-23
• [Presentation] Host plant shoot controls rhizobial symbiotic nitrogen fixation in Lotus japonicus (2013)
  • Author(s): Suganuma, N., Saeki, C., Abo, M., Nishida, H., Handa, Y., Shigenobu, S. and Kawaguchi, M.
  • Organizer: 18^<th> International Congress on Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 2013-10-18
• [Presentation] A SNARE protein expressed in vascular tissue affects symbiotic nitrogen fixation in Lotus japonicus nodules (2012)
  • Author(s): Hakoyama, T., Oi, R., Hazuma, K., Suga, E., Adachi, Y., Kobayashi, M., Akai, R., Sato, S., Fukai, E., Tabata, S., Shibata, S., Wu, G.J., Hase, Y., Tanaka, A., Kawaguchi, M., Kouchi, H., Umehara, Y., and Suganuma, N.
  • Organizer: XV International Congress of Plant-Microbe Interactions
  • Place of Presentation: Kyoto
  • Year and Date: 20120329-0802
• [Presentation] SNAREタンパク質LjSYP71が欠損したミヤコグサ共生変異体 (2011)
  • Author(s): 箱山雅生, 多井諒, 弭間和哉, 須賀江里, 足達由佳, 小林麻由美, 佐藤修正, 深井英吾, 田畑哲之, 柴田哲, 呉国江, 長谷純宏, 田中淳, 川口正代司, 河内宏, 梅原洋佐, 菅沼教生
  • Organizer: 第21回植物微生物研究交流会
  • Place of Presentation: 岡山大学
  • Year and Date: 2011-09-21