2013 Fiscal Year Final Research Report
Molecular characterization of regulatory genes for nicotine biosynthesis in tobacco
| Project/Area Number |
23570055
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
SHOJI Tsubasa 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40343272)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 転写因子 / アルカロイド / タバコ / ジャスモン酸 |
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| Research Abstract |
Transcription factors (TFs) recognize target DNA sequences with distinct DNA-binding domains (DBDs). The DBD of Arabidopsis (Arabidopsis thaliana) ETHYLENE RESPONSE FACTOR1 (AtERF1) uses three consecutive beta-strands to recognize a GCC-containing sequence, but tobacco (Nicotiana tabacum) ERF189 and periwinkle (Catharanthus roseus) Octadecanoid-derivative Responsive Catharanthus AP2-domain protein3 (ORCA3) of the same TF subgroup appear to target similar but divergent DNA sequences. Here, we examined how DNA-binding specificities of these TFs have diverged in each plant lineage to regulate distinct defense metabolisms. Extensive mutational analyses of these DBDs suggest that two modes of protein-DNA interactions independently contribute to binding specificity and affinity Divergent DNA-binding specificities of the ERFs may have arisen through mutational changes of these amino acid residues.
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