2013 Fiscal Year Final Research Report
Analysis of signal transduction induced by compatible signals in the Brassicaceae
| Project/Area Number |
23570056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
IWANO Megumi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50160130)
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| Project Period (FY) |
2011 – 2013
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| Keywords | アブラナ科植物 / カルシウムイオン / 和合受粉 / Calcium ATPase |
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| Research Abstract |
In the Brassicaceae, intraspecific non-self pollen (compatible pollen) can germinate and grow into stigmatic papilla cells, while self pollen or interspecific pollen is rejected at this stage. However, the mechanisms underlying this selective acceptance of compatible pollen remain unclear. Here, using a cell-impermeant calcium indicator, we showed that the compatible pollen coat contains signaling molecule(s) that stimulate Ca2+ export from the papilla cells. Transcriptome analyses of stigmas suggested that autoinhibited Ca2+-ATPase 13 (ACA13) was induced after both compatible pollination and compatible pollen coat treatment. ACA13 protein localized to the plasma membrane. The stigma of a T-DNA insertion line of ACA13 exhibited reduced Ca2+ export, as well as defects in compatible pollen germination and seed production. These findings suggest that stigmatic ACA13 functions in the export of Ca2+ to the compatible pollen tube, which promotes successful fertilization.
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[Journal Article] A pollen coat-inducible autoinhibited Ca^<2+>-ATPase expressed in stigmatic papilla cells is required for compatible pollination in the Brassicaceae2014
Author(s)
Iwano, M., Igarashi, M., Tarutani, Y., Kaothien-Nakayama, P., Nakayama, H., Moriyama, H., Yakabe, R., Entani, T., Shimosato-Asano, H., Ueki, M., Tamiya , G., and Takayama, S.
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Journal Title
Plant Cell
Volume: 26 (2)
Pages: 636-649
DOI
Peer Reviewed
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[Journal Article] Ca^<2+>-activated reactive oxygen species production by Arabidopsis RbohH and RbohJ is essential for proper pollen tube tip growth2014
Author(s)
Kaya, H., Nakajima, R., Iwano, M., Kanaoka, M., Kimura, S., Takeda, S., Kawarazaki, T., Senzaki, E., Hamamura, H., Higashiyama, T., Takayama, S., Abe, M., and Kuchitsu, K.
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Journal Title
Plant Cell
Volume: 26 (3)
Pages: 1069-1080
DOI
Peer Reviewed
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[Journal Article] Physiological and Genetic Analysis of CO_2-Induced Breakdown of Self-Incompatibility in Brassica rapa2014
Author(s)
Lao, X., Suwabe, K., Niikura, S., Kakita, M., Iwano, M., and Takayama, S.
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Journal Title
J Exp Bot.
Volume: 65 (4)
Pages: 939-951
DOI
Peer Reviewed
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[Journal Article] Cell Type-Specific Transcriptome of Brassicaceae Stigmatic Papilla Cells from a Combination of Laser Microdissection and RNA Sequencing2013
Author(s)
Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M.
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Journal Title
Plant Cell Physiol.
Volume: 54 (11)
Pages: 1894-1906
DOI
Peer Reviewed
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[Journal Article] Cytoplasmic Ca^<2+> changes dynamically during the interaction of the pollen tube with synergid cells2012
Author(s)
Iwano, M., Ngo, Q.A., Entani, T., Shiba, H., Nagai, T., Miyawaki, A, Isogai, A, Grossniklaus, U. and Takayama, S.
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Journal Title
Development
Volume: 139 (22)
Pages: 4202-4209
DOI
Peer Reviewed
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[Journal Article] HMG domain containing SSRP1 is required for DNA demethylation and genomic imprinting in Arabidopsis2011
Author(s)
Ikeda, Y., Kinoshita, Y., Susaki, D., Ikeda, Y., Iwano, M., Takayama, S., Higashiyama, T., Kakutani, T. and Kinoshita, T.
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Journal Title
Dev Cell.
Volume: 21 (3)
Pages: 589-596
DOI
Peer Reviewed
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[Journal Article] Glycosylation regulates specific induction of rice immune responses by Acidovorax avenae flagellin2011
Author(s)
Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S. Isogai, A., and Che, F. S.
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Journal Title
J. Biol. Chem.
Volume: 286 (29)
Pages: 25519-25530
DOI
Peer Reviewed
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[Journal Article] Activity of the C-terminal-dependent vacuolar sorting signal of horseradish peroxidase C1a is enhanced by its secondary structure2011
Author(s)
Matsui, T., Tabayashi, A., Iwano, M., Shinmyo, A., Kato, K., and Nakayama, H.
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Journal Title
Plant Cell Physiol.
Volume: 52 (2)
Pages: 413-420
DOI
Peer Reviewed
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[Presentation] アブラナ科植物の自家不和合性の分子機構2012
Author(s)
岩野恵, 伊藤花菜江, 浅野(下里)裕子, 小池千恵子, 高山誠司
Organizer
第83回日本動物学会関連集会「受精研究の現在とこれから〜普遍性と多様性、そして進化の解明を目指して」
Place of Presentation
大阪大学(大阪)
Year and Date
2012-09-13
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