Identification of a plant molecule involved in a surveillance system for the symbiotic nitrogen fixation and its localization in the symbiotic tissues

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kagoshima University
• Principal Investigator: UCHIUMI Toshiki 鹿児島大学, 理工学研究科, 教授 (20193881)
• Project Period (FY): 2011 – 2013
• Keywords: 植物微生物相互作用・共生 / ミヤコグサ / 根粒菌 / 共生窒素固定 / リポ多糖 / リポ多糖結合タンパク質 / 形質転換 / 毛状根

Research Abstract

Lipopolysaccharide (LPS) of bacteria induces the plant resistance in pathogenic combination and is also involved in the establishment of symbiosis between legumes and rhizobia. However, LPS recognition system is still unknown in plants. Referring LBP of mammals, four LBP-candidates were identified on the genome of Lotus japonicus and referred to as LjLBPs. N-terminal barrel of LjLBPs bound with LPS of Escherichia coli. Hairy roots transformed with the fusion genes of LjLBPs and mOrange revealed the localization of LjLBPs. LjLBPs were secreted to the outside of the roots and, in the nodule, targeted to the rhizobia that penetrated into the host plant cells. We investigated the symbiotic phenotype of the hairy roots carrying RNAi- and overexpressing- constructs, and mutant lines generated by EMS treatment or LORE1 insertion. However, we could not establish the idea that LjLBPs were essential for the symbiosis between L. japonicus and its microsymbiont Mesorhizobium loti.

Research Products

• [Journal Article] Characterization of nitric oxide- inducing Lipid A derived from Mesorhizobium loti lipopolysaccharide (2012)
  • Author(s): Hashimoto M, Tanishita Y, Suda Y, Murakami E, Nagata M, Kucho K, Abe M, Uchiumi T.
  • Journal Title: Microbes and Environments Volume: 27 Pages: 490-496
  • Peer Reviewed
• [Journal Article] Nitric oxide production induced in roots of Lotus japonicus by lipopoly- saccharide from Mesorhizobium loti (2011)
  • Author(s): Murakami E, Nagata M, Shimoda Y, Kucho K, Higashi S, Abe M, Hashimoto M, Uchiumi T.
  • Journal Title: Plant and Cell Physiology Volume: 52 Pages: 610-617
  • Peer Reviewed
• [Presentation] 植物の共生:根粒共生系における宿主植物の共生菌飼い馴らし戦略 (2013)
  • Author(s): 内海俊樹
  • Organizer: 第29回日本微生物生態学会大会シンポジウム共生の生物学〜微生物との素敵な出会い
  • Place of Presentation: 鹿児島
  • Year and Date: 20131123-24
  • Invited
• [Presentation] Class 1 plant hemoglobin supports infection process of rhizobia by modulating nitric oxide (2013)
  • Author(s): Kado T, Osuki K, Hashimoto M, Magata M, Murakami E, Tanishita Y, Suda Y, Kucho K, Abe M, Higashi S, Uchiumi T.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎
  • Year and Date: 20131014-18
• [Presentation] Characterization of O-polysaccharide derived from Mesorhizobium loti lipopolysaccharide (2013)
  • Author(s): Hashimoto M, Mizukami M, Suda Y, Kucho K, Abe M, Uchiumi T.
  • Organizer: 18th International Congress on Nitrogen Fixation
  • Place of Presentation: 宮崎
  • Year and Date: 20131014-18
• [Presentation] Mutation of class 1 hemoglobin affects the infection of Mesorhizobium loti to its host plant Lotus japonicus (2012)
  • Author(s): Kado T, Osuki K, Kucho K, Abe M, Higashi S, Uchiumi T.
  • Organizer: The 2nd Asia Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 20121028-31
• [Presentation] Nitric oxide and c lass 1 hemoglobin in the symbiosis between Mesorhizobium loti and Lotus japonicus (2012)
  • Author(s): Kado T, Osuki K, Hashimoto M, Nagata M, Murakami E, Tanishita Y, Suda Y, Kucho K, Abe M, Higashi S, Uchiumi T.
  • Organizer: The 2nd Asia Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Phuket, Thailand
  • Year and Date: 20121028-31
  • Invited
• [Presentation] Class 1ヘモグロビンの変異はMesorhizobium lotiの宿主植物Lotus japonicusへの感染に影響する (2012)
  • Author(s): 角友博, 小薄健一, 九町健一, 阿部美紀子, 東四郎, 内海俊樹
  • Organizer: 植物微生物研究会
  • Place of Presentation: 神戸
  • Year and Date: 20120925-27
• [Presentation] Mutation of class 1 hemoglobin affects the infection of Mesorhizobium loti to its host plant Lotus japonicus (2012)
  • Author(s): Kado T, Osuki K, Kucho K, Abe M, Higashi S, Uchiumi T.
  • Organizer: 10th European Nitrogen Fixation Conference
  • Place of Presentation: Munich, Germany
  • Year and Date: 20120902-05
• [Presentation] Characterization of NO-inducing lipid A from Mesorhizobium loti lipopolysaccharide (2012)
  • Author(s): Hashimoto M, Tanishita Y, Suda Y, Murakami E, Nagata M, Kucho K, Abe M, Uchiumi T.
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都
  • Year and Date: 20120729-0802
• [Presentation] Mutation of class 1 hemoglobin affects the infection of Mesorhizobium loti to its host plant Lotus japonicus (2012)
  • Author(s): Kado T, Osuki K, Kucho K, Abe M, Higashi S, Uchiumi T.
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都
  • Year and Date: 20120729-0802
• [Presentation] Characterization of NO-inducing Lipid A from Mesorhizobium loti lipopolysaccharide (2012)
  • Author(s): Hashimoto M, Tanishita Y, Suda Y, Murakami E, Nagata M, Kucho K, Abe M, Uchiumi T.
  • Organizer: American Society for Microbiology 112th General Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 20120616-19
• [Presentation] Cysteine rich peptides as host-derived molecules that regulate microsymbionts (2012)
  • Author(s): Uchiumi T.
  • Organizer: One day Workshop in"Lessons from interaction between plant and microbe : strategies in symbiosis and competition."
  • Place of Presentation: Kobe University Brussels European Centre, Brussels, Brussels Belgium
  • Year and Date: 2012-11-12
  • Invited
• [Presentation] 根粒着生オートレギュレーションとβ-1,3- glucanase遺伝子(LjGlu1)の発現 (2012)
  • Author(s): 蘭正人, 小薄健一, 鈴木章弘, 原仁俊, 山下健司, 石原真美, 小林優子, 浅見忠男, 九町健一, 東四郎, 阿部美紀子, 内海俊樹
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-16
• [Presentation] Lipopolysaccharide binding protein, a new requirement for legume-rhizobium symbiosis (2011)
  • Author(s): Murakami E, Takayama H, Osuki K, Nagata M, Hori T, Shigeoka M, Sahara M, Muto S, Nagano Y, Kucho K, Abe M, Sato S, Higashi S, Uchiumi T.
  • Organizer: 17th International Congress on Nitrogen Fixation
  • Place of Presentation: Fremantle, Australia
  • Year and Date: 2011-11-29
• [Presentation] Characterization of NO-inducing Lipid A from Mesorhizobium loti lipopolysaccharide (2011)
  • Author(s): Hashimoto M, Tanishita Y, Suda Y, Murakami E, Nagata M, Kucho K, Abe M, Uchiumi T.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] 微生物との共生における植物ヘモグロビンの生理機能 (2011)
  • Author(s): 角友博, 谷村陽一郎, 九町健一, 阿部美紀子, 東四郎, 内海俊樹
  • Organizer: 植物微生物研究会第21回研究交流会
  • Place of Presentation: 岡山
  • Year and Date: 2011-09-21
• [Presentation] Structure and NO-inducing activity of lipopolysaccharide from Mesorhizobium loti (2011)
  • Author(s): Hashimoto M, Tanishita Y, Suda Y, Murakami E, Nagata M, Kucho K, Abe M, Uchiumi T.
  • Organizer: IUMS 2011 XIII International Congress of Bacteriology and Applied Microbiology
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-07
• [Presentation] マメ科植物の根粒菌制御機構 (2011)
  • Author(s): 内海俊樹
  • Organizer: 第17回インターゲノミクスセミナー
  • Place of Presentation: 神戸大学
  • Year and Date: 2011-07-12
  • Invited
• [Presentation] 根粒菌リポ多糖の植物根に対する一酸化窒素産生誘導能とその構造 (2011)
  • Author(s): 橋本雅仁, 谷下洋平, 隅田泰生, 村上英一, 永田真紀, 下田宜司, 東四郎, 九町健一, 阿部美紀子, 内海俊樹
  • Organizer: 第20回内毒素・LPS研究会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-25
• [Presentation] 根粒菌との共生に関与するミヤコグサのリポ多糖結合性タンパク質 (2011)
  • Author(s): 村上英一, 高山仁美, 重岡麻由美, 佐原麻菜, 橋本雅仁, 九町健一, 武藤さやか, 永野幸生, 阿部美紀子, 東四郎, 内海俊樹
  • Organizer: 日本動物学会・日本植物学会・日本生態学会九州支部地区合同長崎大会
  • Place of Presentation: 長崎
  • Year and Date: 2011-05-22
• [Remarks] 植物微生物研究室ホームページ
  • URL: http://www/sci.kagoshima-u.ac.jp/~uttan/top.html