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2013 Fiscal Year Final Research Report

The analysis of movement ability of Ralstonia solanacearum in planta

Research Project

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Project/Area Number 23570079
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionHiroshima University

Principal Investigator

FUJIE MAKOTO  広島大学, 先端物質科学研究科, 准教授 (20274110)

Research Collaborator 梶田 造未  広島大学, 先端物質科学研究科, 学生
磯崎 里奈  広島大学, 先端物質科学研究科, 学生
Project Period (FY) 2011-04-28 – 2014-03-31
Keywords青枯病菌 / エフェクター / 微生物相互作用 / BY-2
Research Abstract

The experimental system to analyze behaviors Ralstonia solanacearum in planta by imaging was established. Furthermore, its attention was paid to the interaction between R. solanacearum and tobacco cultured cell BY-2, as a model of a solanaceous plants. As a result of investigating disease response genes, etc., it was confirmed that BY-2 cells functions as a model system. To study the roles of the effectors in this system, pRES-TG, a shuttle vector between E. coli, was constructed.

  • Research Products

    (13 results)

All 2014 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Characterization of Bacteriophages Cp1 and Cp2, the Strain-Typing Agents for Xanthomonas axonopodis pv. citri2014

    • Author(s)
      Abdelmonim Ali Ahmad, Megumi Ogawa, Takeru Kawasaki, Makoto Fujie and Takashi Yamada
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 80 Pages: 77-85

    • DOI

      10.1128/AEM.02310-13

    • Peer Reviewed
  • [Journal Article] Insights into the diversity ofφRSM phages infecting strains of the phytopathogen Ralstonia solanacearum complex : regulation and evolution2014

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie, Takashi Yamada
    • Journal Title

      Mol Genet Genomics

    • DOI

      10.1007/s00438-014-0835-3

    • Peer Reviewed
  • [Journal Article] The Filamentous PhageφRSS1 Enhances Virulence of Phytopathogenic Ralstonia solanacearum on Tomato2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Pages: 241-245

    • DOI

      10.1094/PHYTO-10-11-0277

    • Peer Reviewed
  • [Journal Article] Loss of Virulence of the Phytopathogen Ralstonia solanacearum Through Infection byφRSM Filamentous Phages2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      PHYTOPATHOLOGY

      Volume: Vol. 102 Pages: 469-477

    • DOI

      10.1094/PHYTO-11-11-0319-R

    • Peer Reviewed
  • [Journal Article] Utilization of Filamentous PhageφRSM3 to Control Bacterial Wilt Caused by Ralstonia solanacearum2012

    • Author(s)
      Hardian S. Addy, Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Plant Disease

      Volume: Vol. 96 Pages: 1204-1209

    • DOI

      10.1094/PDIS-12-11-1023-RE

    • Peer Reviewed
  • [Journal Article] Resolvase-like serine recombinase mediates integration/excision in the bacteriophageφRSM2011

    • Author(s)
      Ahmed Askora, Takeru Kawasaki, Makoto Fujie, and Takashi Yamada
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: VOL. 111 Pages: 109–116

    • DOI

      10.1016/j.jbiosc.2010.10.001

    • Peer Reviewed
  • [Journal Article] Biocontrol of Ralstonia solanacearum by treatment with lytic bacteriophages2011

    • Author(s)
      Fujiwara A, Fujisawa M, Hamasaki R, Kawasaki T, Fujie M, Yamada T.
    • Journal Title

      Appl Environ Microbiol

      Volume: 77 Pages: 4155-62

    • DOI

      10.1128/AEM.02847-10

    • Peer Reviewed
  • [Presentation] タバコ培養細胞BY-2と植物病原菌の相互作用の解析2013

    • Author(s)
      藤江誠, 磯崎里奈, 川崎健, 山田隆
    • Organizer
      日本植物学会第77回大会
    • Place of Presentation
      北海道大学札幌キャンパス
    • Year and Date
      2013-09-15
  • [Presentation] タバコ培養細胞BY-2を利用した青枯病菌感染モデル系の開発2012

    • Author(s)
      磯崎里奈, 梶田浩未, 川崎健, 山田隆, 藤江誠
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学書写キャンパス
    • Year and Date
      2012-09-15
  • [Presentation] 細胞壁分解酵素に着目した青枯病菌の感染メカニズム解明2011

    • Author(s)
      梶田浩未, 藤原亜希子, 川崎健, 藤江誠, 山田隆
    • Organizer
      本生物工学会
    • Place of Presentation
      東京農工大学
    • Year and Date
      2011-09-27
  • [Presentation] Monitoring the behavior of Ralstonia solanacearum cells by GFP labeling during infection process to plant cells2011

    • Author(s)
      Makoto FUJIE, Rina ISOZAKI, Hiromi Kajita, Hirofumi TAKAMOTO, Takeru KAWASAKI, Takashi YAMADA
    • Organizer
      APS(米国植物病理学会)年会
    • Place of Presentation
      ハワイコンベンションセンター(米国)
    • Year and Date
      2011-08-08
  • [Remarks]

    • URL

      http://home.hiroshima-u.ac.jp/mbiotech/ichikou/

  • [Patent(Industrial Property Rights)] 青枯病予防剤および青枯病の予防方法2011

    • Inventor(s)
      山田 隆, 藤江 誠, 川崎 健
    • Industrial Property Rights Holder
      広島大学
    • Industrial Property Rights Type
      特許特開2012-231731
    • Patent Publication Number
      特開2012-231731
    • Filing Date
      2011-04-28
    • Overseas

URL: 

Published: 2015-07-16  

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