Signal transduction mechanism of amino acid response and the modulation of it's responsiveness in newt olfactory receptor neurons.

2015 Fiscal Year Final Research Report

• Project/Area Number: 23570088
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal physiology/Animal behavior
• Research Institution: University of Tsukuba
• Principal Investigator: NAKATANI Kei 筑波大学, 生命環境系, 教授 (20125040)
• Project Period (FY): 2011-04-28 – 2016-03-31
• Keywords: 嗅細胞 / イモリ / アミノ酸応答 / シグナル伝達 / パッチクランプ法

Outline of Final Research Achievements

Newt olfactory receptor neurons respond not only to volatile odorants but also amino acids. However, the molecular mechanism of the signal transduction pathway is not known. In this study, we employed electrophysiological techniques including patch clamp recording and immunohistochemical techniques to elucidate the signal transduction mechanism of amino acid response. The ciliary olfactory receptor neurons responded to both volatile odorants and amino acids. We then applied activators of candidate signal transduction machinery. The results suggest that the signal transduction pathway of amino acid response in ciliary ORN includes both cAMP pathway and IP3 pathway.

Free Research Field

動物生理学