2013 Fiscal Year Final Research Report
Role of myosin-binding protein-C for muscle contraction
| Project/Area Number |
23570089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Chiba University |
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Principal Investigator |
SATO Naruki 千葉大学, 融合科学研究科(研究院), 講師 (40261896)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 動物生理化学 / 筋収縮制御 / ミオシン結合タンパク質 / アクチン / 原索動物 |
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| Research Abstract |
Myosin-binding protein-C (MyBP-C/ C-protein) is one of the major myosin binding proteins and has three isoforms (cardiac, fast-skeletal, slow-skeletal) in vertebrate striated muscles.
In this study, I prepared N-terminal domains encoding mouse cardiac MyBP-C (C0 to C3), that of fast-skeletal MyBP-C (F1 to F4) and that of slow-skeletal MyBP-C (S1 to S4) in an E. coli expression system. My results showed that N-terminal domains of three MyBP-C isoforms cross-linked (or bundled) actin filaments and bound to myosin filaments. The N-terminus of fast skeletal MyBP-C also elevated actin-activated myosin ATPase activity significantly. Furthermore, I identified expression of protochordate MyBP-C in cardiac muscle of Ciona intestinalis. The ciona MyBP-C bound to actin filaments and myosin filaments at the N-terminal and C-terminal region, respectively, as well as vertebrate MyBP-C.
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