Evolutional and phylogenetic analysis of the symbiosis between hydra and chlorella

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biodiversity/Systematics
• Research Institution: Kyushu University
• Principal Investigator: KOBAYAKAWA Yoshitaka 九州大学, 基幹教育院, 教授 (20153588)
• Co-Investigator(Kenkyū-buntansha): TACHIDA Hidenori 九州大学, 理学研究院, 教授 (70216985)
• Project Period (FY): 2011 – 2013
• Keywords: symbiosis / green hydra / chlorella / brown hydra / chlorococcum

Research Abstract

We reconstructed molecular phylogenetic trees of several strains of symbiotic chlorella harbored in the endodermal epithelial cells of viridissima group hydra strains and investigated their congruence with the molecular phylogenetic trees of the host hydra strains. We also confirmed the vertical transition of the symbiotic chlorella through the both reproduction process, asexual budding and the oogenesis in the sexual reproduction. Based on the results, we proposed following hypothesis; Symbiosis with chlorella occurred once in ancestral viridissima group Hydra species, and once established, symbiosis has been conservative in nature and has continued over a long term with occasional cospeciation. We also suspected that the symbiosis between hydra and chlorella have its origin in the age before the breakup of Pangaea.

Research Products

• [Journal Article] Symbiosis between hydra and chlorella : Molecular phylogenetic analysis and experimental study provide insight into its origin and evolution (2013)
  • Author(s): Kawaida, Hitomi; Ohba, Kohki; Koutake, Yuhki; Shimizu, Hiroshi; Tachida, Hidenori; Kobayakawa Yoshitaka.
  • Journal Title: MOLECULAR PHYLOGENETICS AND EVOLUTION Volume: 66 Pages: 906-914
  • DOI: 10.1016/j.ympev.2012.11.018
  • Peer Reviewed
• [Presentation] VERTICAL TRANSMISSION OF SYMBIOTIC CHLORELLAEIN GREEN HYDRA. (2013)
  • Author(s): YAMAGUCHI, J. AND KOBAYAKAWA, Y.
  • Organizer: International Workshop"Unravelling the Developmental Regulatory Network in Early Animals
  • Place of Presentation: Evangelische Akademie Tutzing, Germany
  • Year and Date: 20130900
• [Presentation] Population structure of Hydra magnipapillata within and between ponds -s a pond population clonal or not?- (2012)
  • Author(s): Ohba, K., Tachida, H., Kobayakawa,Y.
  • Organizer: 日本生態学会第59回全国大会
  • Place of Presentation: 大津
  • Year and Date: 20120300
• [Presentation] Molecular phylogenetic study in genus Hydra (2011)
  • Author(s): Kawaida H, Ohba K., Shimizu H, Fujisawa T, Tachida H, and Kobayakawa Y.
  • Organizer: International Workshop"Searching for Eve : basal metazoans and the evolution of multicellular complexity"
  • Place of Presentation: Evangelische Akademie Tutzing, Germany
  • Year and Date: 20110900
• [Presentation] Molecular phylogenetic study on green hydra and its symbionts, Chlorella species (2011)
  • Author(s): Ohba K., Koutake Y., Kawaida H., Shimizu H., Tachida H., and Kobayakawa Y.
  • Organizer: International Workshop"Searching for Eve : basal metazoans and the evolution of multicellular complexity"
  • Place of Presentation: Evangelische Akademie Tutzing, Germany
  • Year and Date: 20110900
• [Presentation] クロレラの再導入実験による宿主グリーンヒドラと共生クロレラの種特異性についての検討 (2011)
  • Author(s): 神武祐紀、小早川義尚
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 20110900
• [Presentation] グリーンヒドラの配偶子形成過程におけるmRNA5'Cap metyl-transferase(CMet )の発現 (2011)
  • Author(s): 笹原大輔、小早川義尚
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 20110900
• [Presentation] ヒドラの刺胞芽細胞特異的に発現する新規遺伝子について (2011)
  • Author(s): 小川耕佑、小早川義尚
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 20110900
• [Presentation] グリーンヒドラの触手過剰変異体の解析 (2011)
  • Author(s): 山口淳也、小早川義尚
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 20110900