Development of basic glycotechnology for construction of multifunctional proteoglycan molecules.

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570157
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: IKUKO Kakizaki 弘前大学, 医学(系)研究科(研究院), 准教授 (80302024)
• Project Period (FY): 2011 – 2013
• Keywords: 糖鎖 / 糖鎖工学

Research Abstract

Hydrolysis and transglycosylation reactions of hyaluronidase (from bovine testes) and cellulase (from A. nigar) were investigated with the aim of applying them in glycotechnology for proteoglycans. As reaction substrates, commercially available sugar chains in addition to proteoglycans, as well as oligosaccharides having linkage structures between a core peptide and a sugar chain of proteoglycan, and de-sulfated sugar chains that we prepared, were used. We found a sugar cahin structure that was degraded by hyaluronidase but possibly did not act as a donor substrate for the transglycosylation reaction. The optimal condition for proteoglycan hydrolysis by cellulase was determined, however, transglycosylation reaction could not be detected.

Research Products

• [Journal Article] One set system for the synthesis and purification of glycosaminoglycan oligosaccharides reconstructed using a hyaluronidase-immobilized column (2014)
  • Author(s): Suto, S., Kakizaki, I., Nakamura, T., and Endo, M.
  • Journal Title: Biopolymers Volume: 101(3) Pages: 189-96
  • DOI: 10.1002/bip.22300
  • Peer Reviewed
• [Journal Article] Custom synthesis of hyaluronan/chondroitin sulfate hybrid oligosaccharides : for future medical applications, in The 13^<th> Meeting of Hirosaki International Forum of Medical Science"Innovation in Transplant and Regenerative Medicine" (2013)
  • Author(s): Kakizaki, I., Suto, S., Tatara, Y., and Endo, M.
  • Journal Title: Hirosaki Med. J. Volume: 64(Suppl) (edited by Oyama, C., et al) Pages: 53–57
  • URL: http://repository.ul.hirosaki-u.ac.jp/dspace/handle/10129/4866
• [Journal Article] Hyaluronan-chondroitin hybrid oligosaccharides as new life science research tools (2012)
  • Author(s): Kakizaki, I., Suto, S., Tatara, Y., Nakamura, T., and Endo, M.
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 423(2) Pages: 344-349
  • DOI: 10.1016/j.bbrc.2012.05.127
  • Peer Reviewed
• [Journal Article] Interaction of Listeria monocytogenes autolysin amidase with glycosaminoglycans promotes listerial adhesion to mouse hepatocytes (2012)
  • Author(s): Asano, K., Kakizaki, I., and Nakane, A.
  • Journal Title: Biochimie Volume: 94(6) Pages: 1291-1299
  • DOI: 10.1016/j.biochi.2012.02.026
  • Peer Reviewed
• [Journal Article] Anti-inflammatory effect of proteoglycan and progesterone on human uterine cervical fibroblasts (2012)
  • Author(s): Fukuyama, A., Tanaka, K., Kakizaki, I., Kasai, K., Chiba, M., Nakamura, T., and Mizunuma, H.
  • Journal Title: Life Sci. Volume: 90(13-14) Pages: 484-488
  • DOI: 10.1016/j.lfs.2011.12.024
  • Peer Reviewed
• [Journal Article] Synthesis of neoproteoglycans using the transglycosylation reaction as a reverse reaction of endo-glycosidases (2012)
  • Author(s): Endo, M., and Kakizaki, I.
  • Journal Title: Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. Volume: 88(7) Pages: 327-344
  • URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3422686/.Review
  • Peer Reviewed
• [Presentation] Enzymatic synthesis of urinary trypsin inhibitor having an O-linked hyaluronan chain (2013)
  • Author(s): Kakizaki, I., Takahashi, R., Yanagisawa, M., Yoshida, T., and Takagaki, K.
  • Organizer: 9^<th>International Conference on Hyaluronan (Hyaluronan 2013)
  • Place of Presentation: Oklahoma city, OK, USA
  • Year and Date: 20130602-07
• [Presentation] 酵素を用いたグリコサミノグリカン糖鎖の構造解析 (2013)
  • Author(s): 石岡陽菜,柿崎育子,多田羅洋太,須藤晋一郎,遠藤正彦
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-09-11
• [Presentation] ヒアルロニダーゼ固定化酵素を利用した人工オリゴ糖の合成と精製システム (2012)
  • Author(s): 須藤晋一郎,柿崎育子,多田羅洋太,中村敏也,遠藤正彦
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-15
• [Presentation] 日本応用糖質科学会平成23年度大会(第60回)大会特別シンポジウム【応用糖質科学研究の新時代を拓く】 (2011)
  • Author(s): 柿崎育子
  • Organizer: 機能解析モデルとしての糖鎖改変プロテオグリカン
  • Place of Presentation: 札幌市
  • Year and Date: 2011-09-29
• [Presentation] サケ鼻軟骨由来プロテオグリカンの糖鎖分析 (2011)
  • Author(s): 柿崎育子,峯田貴,佐々木愛,多田羅洋太,牧野英司,遠藤正彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-23
• [Presentation] 人工ハイブリッドヒアルロナンとヒアルロナン結合タンパク質の結合 (2011)
  • Author(s): 須藤晋一郎,柿崎育子,多田羅洋太,中村敏也,遠藤正彦
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-23
• [Book] サケ鼻軟骨由来のプロテオグリカン『マリンバイオテクノロジーの新潮流』 (2011)
  • Author(s): 加藤陽治, 柿崎育子
  • Total Pages: 129-140
  • Publisher: シーエムシー出版刊
• [Remarks]
  • URL: http://www.med.hirosaki-u.ac.jp/~bioche1/