2013 Fiscal Year Final Research Report
Understanding of the regulatory mechanism on the activity of chloroplast-type ATP synthase by X-ray crystallography
| Project/Area Number |
23570159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Kanazawa University |
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Principal Investigator |
KONNO HIROKI 金沢大学, バイオAFM先端研究センター, 准教授 (80419267)
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| Project Period (FY) |
2011 – 2013
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| Keywords | ATP合成酵素 / 葉緑体 |
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| Research Abstract |
Inserted region on the gamma subunit of chloroplast type ATP synthase strongly affect to the inhibitory effect of endogenous epsilon subunit (epsilon inhibition). To understand the relationship between the inserted region and the epsilon subunit, we try to dissolve structure of sub-complex of chloroplast type ATP synthase (alpha3beta3gamma1epsilon1). Though we could not yet get crystal in high resolution, we succeeded to make stable sub-complex mutant for crystallization and could get some crystal.
We also investigated an affect of the inserted region on the gamma subunit to the ATPase activity on the beta subunit because these two parts exist far form each other. We then identified a conformational change of two central alpha helices in gamma subunit which is interact with catalytic sites on the beta subunit is necessary to read regulatory effect of the inserted region.
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[Journal Article] Common evolutionary origin for the rotor domain of rotary ATPases and flagellar protein export apparatus2013
Author(s)
Kishikawa, J., Ibuki, T., Nakamura, S., Nakanishi, A., Minamino, Y., Miyata, T., Namba, K., Konno, H., Ueno, H., Imada, K., and Yokoyama, K.
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Journal Title
PLoS One
Volume: 8(5)
Pages: 64692013
DOI
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[Journal Article] Nano-scale alignment of proteins on a flexible DNA backbone2012
Author(s)
Nojima, T., Konno, H., Kodera, N., Seio, K., Taguchi, H., Yoshida, M.
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Journal Title
PLoS One
Volume: 7
Pages: 52534
DOI
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[Journal Article] Thiol modulation of the chloroplast ATP synthase is dependent on the energization of thylakoid membranes2012
Author(s)
Konno, H., Nakane, T., Yoshida, M., Ueoka-Nakanishi, H., Hara, S., Hisabori T.
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Journal Title
Plant Cell Physiol
Volume: 53
Pages: 626-34
DOI
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[Journal Article] Regulation of F_oF_1-ATPase from Synechocystis sp. PCC 6803 by theγandεsubunits is significant for light/dark adaptation2011
Author(s)
Imashimizu, M., Bernat, G., Sunamura, EI., Broekmans, M., Konno, H., Isato, K., Roegner, M., Hisabori, T.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 26595-26602
DOI
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[Presentation] Conformational change of theγsubunit regulates the ATP hydrolysis activity of cyanobacterial ATP synthase2012
Author(s)
Sunamura, EI, Konno, H, Imashimizu, M, Mochimaru, M, Hisabori, T.
Organizer
17th European Bioenergetics Conference
Place of Presentation
University of Freiburg, Germany
Year and Date
20120915-20
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