2013 Fiscal Year Final Research Report
The degradation pathway of plant N-glycans and functional analysis of the N-glycan degradation-deficient mutant
| Project/Area Number |
23570163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Ritsumeikan University (2012-2013)
Osaka University (2011) |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2013
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| Keywords | 糖タンパク質 / N型糖鎖 / 糖質関連酵素 / フコシダーゼ / フコース / 植物 |
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| Research Abstract |
The degradation pathway of high-mannose type N-glycans in plant cells has been clarified. In this study, we identified a gene encoding (alpha)1,3-fucosidase acting on Fuc(alpha)1-3GlcNAc linkage in plant complex type N-glycans. Its substrate specificity analysis revealed the degradation pathway of plant complex type N-glycans. The (alpha)1,3-fucosidase-deficient mutant changed the composition of N-glycans in plant cells, showing that (alpha)1,3-fucosidase is an essential glycosidase in plant N-glycan degradation.
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