Function and metabolism of a histidine-containing dipeptide, carnosine, in mammals

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570164
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: OKUMURA Nobuaki 大阪大学, たんぱく質研究所, 准教授 (20224173)
• Project Period (FY): 2011 – 2013
• Keywords: ペプチド代謝 / ジペプチダーゼ / 金属プロテアーゼ / 骨格筋 / 脳

Research Abstract

Carnosine is a histidine-containing dipeptides present at high concentrations in mammalian skeletal muscles and the brain. It has been proposed that they have a variety of biological functions including anti-oxidant, pH-buffering and nutritional activities. Recently, we and other groups have identified the enzymes responsible for carnosine synthesis and degradation, but the function of these enzymes are still to be investigated. In this study, in order to clarify the functional roles of these enzymes, we have analyzed the enzymatic characteristics and cellular distribution of these enzymes, especially focusing on a carnosine-degrading enzyme, carnosine dipeptidase II (CN2).

Research Products

• [Journal Article] Diversity in protein profiles of individual calcium oxalate kidney stones (2013)
  • Author(s): Okumura N, Tsujihata M, Momohara C, Yoshioka I, Suto K, Nonomura N, Okuyama A, Takao T.
  • Journal Title: PLoS One Volume: 8 Pages: 68624
• [Journal Article] Identification of cargo proteins specific for importin-beta with importin-alpha applying a stable isotope labeling by amino acids in cell culture (SILAC)-based in vitro transport system (2013)
  • Author(s): Kimura M, Okumura N, Kose S, Takao T, Imamoto N.
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 24540-24549
• [Journal Article] Identification of cargo proteins specific for the nucleocytoplasmic transport carrier transportin by combination of an in vitro transport system and stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics (2013)
  • Author(s): Kimura M, Kose S, Okumura N, Imai K, Furuta M, Sakiyama N, Tomii K, Horton P, Takao T, Imamoto N.
  • Journal Title: Mol Cell Proteomics Volume: 12 Pages: 145-157
• [Journal Article] Role of L-carnosine in the control of blood glucose, blood pressure, thermogenesis, and lipolysis by autonomic nerves in rats : involvement of the circadian clock and histamine (2012)
  • Author(s): Nagai K, Tanida M, Niijima A, Tsuruoka N, Kiso Y, Horii Y, Shen J, Okumura N.
  • Journal Title: Amino Acids Volume: 43 Pages: 97-109
• [Presentation] ESI-TOF MSを用いたカルノシンジペプチダーゼ2 (CNDP2)のダイマー形成の解析 (2011)
  • Author(s): 田村淳, 高尾敏文, 奥村宣明
  • Organizer: 第59回質量分析討論会
  • Place of Presentation: 大阪
  • Year and Date: 20110913-15
• [Presentation] Requirement of dimer formation for carnosine-hydrolyzing activity of carnosinase CNDP2 (2011)
  • Author(s): Okumura N., Kusunoki, M., Okumura A., Nagai, K., Takao T.
  • Organizer: Poster presentation at International congress on carnosine in exercise and disease
  • Place of Presentation: Belgium
  • Year and Date: 20110700
• [Book] Carnosine dipeptidase II (in Handbook of Proteolytic Enzymes) (2012)
  • Author(s): Okumura N.
  • Total Pages: (分担執筆)
  • Publisher: Academic Press (Waltham, MA)
• [Book] Carnosine and the blood glucose. (in Food and Nutritional Components in Focus)
  • Author(s): Okumura N. Tanida M. Niijima A, Tsuruoka N. Kiso Y. Horii Y. Nagai K.
  • Total Pages: (分担執筆, 編集中)
  • Publisher: RSC publishing (London)
• [Remarks]
  • URL: http://www.protein.osaka-u.ac.jp/metabolism/taisha.html