Perturbation to the electron transfer and distribution due to the connection between ferredoxin and its partner proteins

2014 Fiscal Year Final Research Report

• Project/Area Number: 23570165
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: ARIGA YOKO 大阪大学, たんぱく質研究所, 助教 (60255429)
• Co-Investigator(Kenkyū-buntansha): HASE Toshiharu 大阪大学, 蛋白質研究所, 教授 (00127276)
• Co-Investigator(Renkei-kenkyūsha): KURISU Genji 大阪大学, 蛋白質研究所, 教授 (90294131)
• Research Collaborator: KINOSHITA Misaki
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: 生体エネルギー変換 / 光合成

Outline of Final Research Achievements

Plants maintain life activity by flexibly distributing the energy acquired from the light to a various biosynthesis reactions according to the growth process and the environmental change, but the mechanism of its clever energy distribution isn't clear. In this study, we prepared a series of cross-linked complexes and interaction mutants of ferredoxin, an energy (high energy electron) distributing protein, and its electron acceptor proteins, and their three-dimensional structures by those atomic levels, electron transfer function and protein-protein interaction in vitro and in vivo system were investigated. As a result, several interesting observations leading to novel regulatory mechanisms of interaction between ferredoxin and the electron acceptor proteins were obtained.

Free Research Field

生物学、 生物科学・機能生物化学