Giantin regulates the early secretory pathway by controlling communication among Golgi stacks

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: SATOH AYANO 岡山大学, 自然科学研究科, 准教授 (40303002)
• Project Period (FY): 2011 – 2013
• Keywords: 分泌 / ゴルジ体 / 細胞内輸送 / 糖鎖

Research Abstract

Golgin tethers function in COPI vesicle transport around the Golgi apparatus. Here we show by RNAi that the loss-of-function of a golgin tether, Giantin changed the length of Golgi cisternae. Cargo transport increased in the RNAi cells. Furthermore, surface glycosylation patterns and localization of Golgi enzymes were changed in the RNAi cells. These suggest that golgin tethers may be necessary for Golgi formation and give an accuracy of COPI vesicle transport.

Research Products

• [Journal Article] (2013)
  • Author(s): Koreishi, M., Gniadek, T. J., Yu, S., Masuda, J., Honjo, Y., Satoh, A.
  • Journal Title: PLoS One Volume: 8 Pages: 59821
• [Journal Article] (2013)
  • Author(s): Koreishi, M., Yu, S., Oda, M., Honjo, Y., Satoh, A.
  • Journal Title: PLoS One Volume: 8 Pages: 54382
• [Journal Article] (2013)
  • Author(s): Satoh, A., Iwakiri, Y.
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 25 Pages: 241-244
• [Journal Article] (2013)
  • Author(s): Tashiro, K., Satoh, A., Utsumi, T., Chung, C., Iwakiri, Y.
  • Journal Title: Am J Pathol.
• [Journal Article] (2013)
  • Author(s): Matsuda, S., Yan, T., Mizutani, A., Sota, T., Hiramoto, Y., Prieto-Vila, M., Chen, L., Satoh, A., Kudoh, T., Kasai, T., Murakami, H., Fu, L., Salomon, D. S., Seno, M.
  • Journal Title: Int J Cancer.
• [Journal Article] (2013)
  • Author(s): Sekhar, S. C., Kasai, T., Satoh, A., Shigehiro, T., Mizutani, A., Murakami, H., El-Aarag, B. Y., Salomon, D. S., Massaguer, A., de Llorens, R., Seno, M.
  • Journal Title: J Cancer. Volume: 4 Pages: 391-401
• [Journal Article] (2012)
  • Author(s): Zong, M., Satoh, A., Yu, M. K., Siu, K. Y., Ng, W. Y., Chan, H. C., Tanner, J. A., Yu, S.
  • Journal Title: PLoS One Volume: 7 Pages: 29995
• [Journal Article] (2012)
  • Author(s): Chen, L., Kasai, T., Li, Y., Sugii, Y., Jin, G., Okada, M., Vaidyanath, A., Mizutani, A., Satoh, A., Kudoh, T., Hendrix, M. J., Salomon, D. S., Fu, L., Seno, M.
  • Journal Title: PLoS One Volume: 7 Pages: 33544
• [Journal Article] (2011)
  • Author(s): Koreishi, M., Honjo, Y., Satoh, A.
  • Journal Title: Antibody Technology Journal 2011 Pages: 5-11
• [Journal Article]
  • Author(s): Yamada, H., Abe, T., Satoh, A, Okazaki, N., Tago, S., Kobayashi, K., Yoshida, Y., Oda, Y., Watanabe, M., Tomizawa, K., Matsui, H., Takei, K.
  • Journal Title: J Neurosci. Volume: 33 Pages: 4514-4526
• [Presentation] ゴルジ体形成と分泌におけるゴルジンタンパク質の機能解析 (2013)
  • Author(s): 佐藤あやの
  • Organizer: 第32回日本糖質学会年会
  • Place of Presentation: 大阪国際交流センター
  • Year and Date: 2013-08-05
• [Presentation] The golgin tether Giantin regulates the secretory pathway by controlling stack organization within Golgi apparatus (2013)
  • Author(s): Satoh A.
  • Organizer: 第65回日本細胞生物学会大会
  • Place of Presentation: ウインクあいち(愛知県産業労働センター)
  • Year and Date: 2013-06-19
• [Presentation] The role of CK2 in Sec31 phosphorylation and membrane trafficking (2012)
  • Author(s): A, Sato.
  • Organizer: 第52回アメリカ細胞生物学会年会
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2012-12-15
• [Presentation] Sec31のリン酸化と膜輸送におけるCK2の機能解析 (2012)
  • Author(s): A, Satoh.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場、マリンメッセ福岡
  • Year and Date: 2012-12-11
• [Presentation] Function of Golgin family proteins in trafficking and Golgi biogenesis (2011)
  • Author(s): A, Satoh.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2011-09-21
• [Remarks]
  • URL: http://www.biotech.okayama-u.ac.jp/labs/satoh
• [Patent(Industrial Property Rights)] 細胞死抑制剤及び新規化合物 (2014)
  • Inventor(s): 佐藤あやの・仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの・仁科勇太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2014/55532
  • Filing Date: 2014-03-05
  • Overseas
• [Patent(Industrial Property Rights)] 細胞死抑制剤及びその製造方法 (2014)
  • Inventor(s): 佐藤あやの・仁科勇太
  • Industrial Property Rights Holder: 佐藤あやの・仁科勇太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2014/55533
  • Filing Date: 2014-03-05
  • Overseas
• [Patent(Industrial Property Rights)] Giantinを標的とするRNAi誘導性核酸及び分泌機能改善剤 (2013)
  • Inventor(s): 佐藤あやの
  • Industrial Property Rights Holder: 佐藤あやの
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 特願2012-547615
  • Filing Date: 2013-11-22
  • Overseas