Molecular mechanisms of receptor trafficking by a phosphatidic acid-interacting Rab11 effector and their regulators

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570174
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: INOUE Hiroki 東京薬科大学, 生命科学部, 講師 (10294448)
• Co-Investigator(Renkei-kenkyūsha): TAGAYA Mitsuo 東京薬科大学, 生命科学部, 教授 (30179569)
• Project Period (FY): 2011 – 2013
• Keywords: 小胞輸送 / 上皮成長因子受容体 / トランスフェリン受容体 / SNARE / gamma-SNAP / DDHD2 / KIAA0725p / Rab11-FIP5

Research Abstract

In this study, we revealed that DDHD2/KIAA0725p, phospholipase A1 against phosphatidic acid (PA), interacts with phosphatidylinositol 4-phosphate, thereby being recruited to ERGIC/Golgi. Moreover, this study showed that gamma-SNAP, which is a binding partner of PA-interacting Rab11 effector Rab11-FIP5, functions in disassembly of SNARE complex and regulates the trafficking of epidermal growth factor receptor and transferrin from early endosomes.

Research Products

• [Journal Article] A new role for RINT-1 in SNARE complex assembly at the trans-Golgi network in coordination with the COG complex (2013)
  • Author(s): Arasaki, K., Takagi, D., Furuno, A., Sohda, M., Misumi, Y., Wakana, Y., Inoue, H., and Tagaya, M.
  • Journal Title: Mol. Biol. Cell. Volume: 24 Pages: 2907-2917
  • DOI: 10.1091/mbc.E13-01-0014
• [Journal Article] Roles of SAM and DDHD domains in mammalian intracellular phospholipase A1 KIAA0725p (2012)
  • Author(s): Inoue, H., Baba, T., Sato, S., Ohtsuki, R., Takemori, A., Watanabe, T., Tagaya, M., and Tani, K.
  • Journal Title: Biochim. Biophys. Acta Volume: 1823 (*, These authors contributed equally to this work.) Pages: 930-939
  • DOI: 10.1016/j.bbamcr.2012.02.002
• [Journal Article] The intracellular phospholipase A1 protein family (2012)
  • Author(s): Tani, K., Kogure, T., and Inoue, H.
  • Journal Title: Biomol. Concepts Volume: 3 Pages: 471-478
  • DOI: 10.1515/bmc-2012-0014
• [Presentation] γ-SNAPの結合因子と細胞内小胞輸送における機能 (2012)
  • Author(s): 松崎友香,田中理華,渡邉卓也,井上弘樹,奥崎大介,多賀谷光男
  • Organizer: 第85回日本生化学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121200
• [Presentation] 細胞内型ホスホリパーゼA1のSAM-DDHDドメインはホスファチジルイノシトールリン酸の結合とホスホリパーゼA1活性に必須の領域である (2011)
  • Author(s): 竹森亜弥,井上弘樹,馬場崇,大槻竜也,渡邉卓也,多賀谷光男,谷佳津子
  • Organizer: 第84回日本生化学会年会
  • Place of Presentation: 京都
  • Year and Date: 20110900
• [Book] The Structures and Functions of Intracellular Phospholipase A1 family proteins. In "Phospholipases in Health and Disease" Series : Advances in Biochemistry in Health and Disease (2014)
  • Author(s): Tani, K., Baba, T. and Inoue, H.
  • Total Pages: Vol.10 (87-99)
  • Publisher: Springer Science, Berlin, Germany
• [Remarks]
  • URL: http://www.toyaku.ac.jp/