Study of autonomous oscillation in PEG-crosslinked actin hydrogel

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570181
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Nippon Institute of Technology
• Principal Investigator: SANO Ken-Ichi 日本工業大学, 工学部, 准教授 (80321769)
• Project Period (FY): 2011 – 2013
• Keywords: アクチン / ダイナミックインスタビリティ / ナノバイオ

Research Abstract

We succeeded in synthesizing chemically poly-ethylene glycol cross-linked hydrogels comprised of actin. We have found that the storage modulus of this actin hydrogel autonomously oscillated for more than ten hours. The oscillatory properties of the actin hydrogels were based on both the treadmilling and gelation by chemical crosslinking of the filamentous actin structures. Thus oscillation can be caused by dynamic instability of actin filament. In this study, I developed simultaneous measurement system both mechanical strength and polymerization of actin, and build a model of oscillating actin gel based on dynamic instability. Additionally, exploring the specific motility of the cytoskeletal protein hydrogel when combined with kinesin linear motor protein.

Research Products

• [Journal Article] 細胞骨格トレッドミルマシン—バイオアクチュエータとしての可能性 (2013)
  • Author(s): 川村隆三, 佐野健一, 長田義仁
  • Journal Title: 設計工学バイオサイエンスとインダストリー Volume: 48 Pages: 11-15
• [Journal Article] Self-repairing filamentous actin hydrogel with hierarchical structure (2011)
  • Author(s): Sano K., Kawamura R., Tominaga T., Oda N., Ijiro K., Osada Y.
  • Journal Title: Biomacromolecules Volume: 12 Pages: 4173-4177
  • Peer Reviewed
• [Presentation] Slime mold in conflicting (2013)
  • Author(s): Sano K., Ichimura M., Arai Y., Nakayama N., Hara M., Aono M.
  • Organizer: International Symposium on Advanced Soft Materials
  • Place of Presentation: 札幌市
  • Year and Date: 2013-10-18
• [Presentation] 生物を倣い、材料から習うナノテクノロジー (2013)
  • Author(s): 佐野健一
  • Organizer: 精密工学会第65回マイクロ / ナノシステム研究専門委員会
  • Place of Presentation: 東京都目黒区
  • Year and Date: 2013-10-11
  • Invited
• [Presentation] 葛藤する真正粘菌とその解析 (2013)
  • Author(s): 佐野健一, 新井裕太, 市村充, 中山典久, 原正彦, 青野真士
  • Organizer: 第86回日本生化学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2013-09-11
• [Presentation] Oscillatory property of PEG-Crosslinked Actin Hydrogel (2012)
  • Author(s): Sano K., Kawamura R., Tominaga T., Osada Y.
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-14
• [Presentation] Microtubule hydrogel and the motility with motor protein (2012)
  • Author(s): Kawamura R., Sano K., Tominaga T., Oda N., Ijiro K., Osada Y.
  • Organizer: GelSympo2012
  • Place of Presentation: つくば市
  • Year and Date: 2012-10-10
• [Presentation] 高次階層構造による微小管ゲルのメカノケミカル効果 (2012)
  • Author(s): 川村隆三, 佐野健一, 富永大輝, 小田直子, 居城邦治, 長田義仁
  • Organizer: 第61回高分子討論会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-09-19
• [Presentation] 生体分子を使った静的な構造構築から動的な構造形成へ (2012)
  • Author(s): 佐野健一
  • Organizer: 北海道大学電子科学研究所講演会
  • Place of Presentation: 札幌市
  • Year and Date: 2012-09-10
  • Invited
• [Presentation] 生体分子の分子認識能の利用 (2012)
  • Author(s): 佐野健一
  • Organizer: つくばソフトマター研究会
  • Place of Presentation: 東海村
  • Year and Date: 2012-08-30
  • Invited
• [Presentation] Stress-responsible cytoskeletal protein hydrogels with high hierarchical structure (2011)
  • Author(s): Sano K., Kawamura R., Ijiro K., Osada Y.
  • Organizer: International Conference on Biopolymers 2011
  • Place of Presentation: 北京(中国)
  • Year and Date: 2011-10-19
  • Invited
• [Presentation] 細胞骨格タンパク質の機能材料化 (2011)
  • Author(s): 佐野健一, 川村隆三, 富永大輝, 小田直子, 居城邦治, 長田義仁
  • Organizer: 第60回高分子学会年会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-05-26
• [Book] Employing Cytoskeletal Treadmilling in Bio-Actuator"in Soft Actuators- Materials, Modeling, Applications, and Future Perspectives (2014)
  • Author(s): Sano K., Kawamura R., Osada Y.
  • Total Pages: (印刷中) 10
  • Publisher: Springer