2014 Fiscal Year Final Research Report
Fibrin translocation to platelet membrane rafts in blood coagulation
Project/Area Number |
23570182
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | Tokyo Metropolitan Institute of Medical Science |
Principal Investigator |
KASAHARA Kohji 公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 副室長 (60250213)
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Project Period (FY) |
2011-04-28 – 2015-03-31
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Keywords | ラフト / 血小板 / フィブリン / 血液凝固 |
Outline of Final Research Achievements |
We observed that sphingomyelin (SM)-rich rafts are identified histochemically in the central region of adhered platelets where fibrin is colocalized on activation by thrombin. The clot retraction of SM-depleted platelets from SM synthase knockout mouse was delayed significantly, suggesting that platelet SM-rich rafts are involved in clot retraction. Furthermore, fibrin translocation to DRM rafts was impaired in factor XIII A subunit-deficient mouse platelets, which show impaired clot retraction. Furthermore, the disruption of SM-rich rafts by methyl-β-cyclodextrin impaired clot retraction. Thus, we propose that clot retraction takes place in SM-rich rafts where a fibrin-αIIbβ3 complex is formed as a primary axis to promote platelet contraction.
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Free Research Field |
機能生物化学
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