2013 Fiscal Year Final Research Report

Molecular mechanism for arrested fork stabilization by fork recognition complex

Research Project

Project/Area Number	23570183
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Functional biochemistry
Research Institution	Tokyo Metropolitan Institute of Medical Science
Principal Investigator	TANAKA Taku 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (80425686)
Project Period (FY)	2011 – 2013
Keywords	DNA複製 / 複製起点 / 複製再開始 / マイクロアレイ / PriA / RecA / 複製フォーク / フォーク安定化因子
Research Abstract	Analyses for arrested replication fork recognition by fork stabilizing factor are important for elucidation of molecular mechanism of replication restart reaction. Escherichia coli PriA protein, bacterial fork stabilizing factor which can bind to arrested replication forks, can be used as an indicator for detection of arrested forks in living cells. PriA binds to novel replication origin and replication restart, which is activated by origin-independent system, depends on formation of RNA-DNA hybrid. RNA-dependent initiation system for replication may reflect primitive replication mechanism and these findings shed light on evolution of eukaryotic replication system.