Function of tubulin polyglutamylation in cilia/flagella motility mechanism

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biophysics
• Research Institution: Gakushuin University (2012-2013); The University of Tokyo (2011)
• Principal Investigator: KAMIYA Ritsu 学習院大学, 理学部, 客員教授 (10124314)
• Project Period (FY): 2011 – 2013
• Keywords: 運動・輸送 / 分子モーター / 翻訳後修飾 / 鞭毛・繊毛 / 微小管

Research Abstract

To approach the function of tubulin polyglutamylation in cilia/flagella motility, we examined the motile properties of a Chlamydomonas tpg1, a mutant that lacks a tubulin polygultamylating enzyme (TTLL9). Motility analyses in double mutants between tpg1 and various kinds of dynein-deficient mutants revealed that long-chain tubulin polyglutamylation affects the function of only one particular species of dynein, inner arm dynein e, among more than 10 species of axonemal dyneins. Furthermore, studies using another mutant, tpg2, showed that a protein designated FAP234 functions in the transport and stabilization of TTLL9.

Research Products

• [Journal Article] A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9 (2014)
  • Author(s): Kubo T, Yanagisawa HA, Liu Z, Shibuya R, Hirono M, Kamiya R.
  • Journal Title: Mol. Biol. Cell. Volume: 25 Pages: 107-117
  • DOI: 10.1091/mbc.E13-07-0424
  • Peer Reviewed
• [Journal Article] The MIA complex is a conserved and novel dynein regulator essential for normal ciliary motility (2013)
  • Author(s): Yamamoto, R., Song, K., Yanagisawa, H., Fox, L., Yagi, T., Wirschell, M., Hirono, M., Kamiya, R., Nicastro, D., and Sale, W.S.
  • Journal Title: J. Cell Biol. Volume: 201 Pages: 263-278
  • DOI: 10.1083/jcb.201211048
  • Peer Reviewed
• [Journal Article] Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex (2012)
  • Author(s): Kubo, T., Yagi, T., and Kamiya, R.
  • Journal Title: Cytoskeleton Volume: 69 Pages: 1059-1068
  • DOI: 10.1002/cm.21075
  • Peer Reviewed
• [Journal Article] Polarity and asymmetry in the arrangement of dynein and related structures in the Chlamydomonas axoneme (2012)
  • Author(s): Bui, K.H., Yagi, T., Yamamoto, R., Kamiya, R., and Ishikawa, T.
  • Journal Title: J. Cell Biol. Volume: 198 Pages: 913–925
  • DOI: 10.1083/jcb.201201120
  • Peer Reviewed
• [Presentation] Regulation of axonemal dynein activity by a conserved tubulin polyglutamylation system (2013)
  • Author(s): Ritsu Kamiya.
  • Organizer: International Workshop : Dynein 2014
  • Place of Presentation: オルビスホール(兵庫県神戸市)
  • Year and Date: 20131013-1103
• [Presentation] Regulation of dynein activity by a conserved tubulin polyglutamylation system (2013)
  • Author(s): Ritsu Kamiya.
  • Organizer: The 25th CDB Meeting : Cilia and centrosomes : from fertilization to cancer
  • Place of Presentation: 理化学研究所CDB(兵庫県神戸市)
  • Year and Date: 20130617-18
  • Invited
• [Presentation] 鞭毛運動におけるダイニン機能とその調節機構の研究 (2012)
  • Author(s): 神谷律
  • Organizer: 第83回日本動物学会
  • Place of Presentation: 大阪大学(大阪府豊中市) (学会賞受賞講演)
  • Year and Date: 2012-09-14
• [Book] 太古からの9+2構造:繊毛のなぞ (2012)
  • Author(s): 神谷律
  • Total Pages: 2014
  • Publisher: 岩波書店