2013 Fiscal Year Final Research Report
The differential analyses of tRNA splicing processes among the three domains of life
| Project/Area Number |
23570208
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Satoshi 岐阜大学, 工学部, 助教 (10345796)
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| Research Collaborator |
NOMURA Yuichiro 岐阜大学, 大学院工学研究科, 大学院生
KUNO Miyuki 岐阜大学, 大学院工学研究科, 大学院生
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| Project Period (FY) |
2011 – 2013
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| Keywords | tRNA / tRNAリガーゼ / スプライシング / 古細菌 / EndA / RtcB / イントロン / 低分子環状RNA |
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| Research Abstract |
In Archaea and Eucarya, some tRNA genes contain introns. Since the precursor tRNA (pre-tRNA) is not functional in protein biosynthesis, exact removal of introns must be essential. Recently, it was found that the RtcB catalyzes the ligation of exons derived from the pre-tRNA. RtcB also seems to catalyze the circulation of intron from pre-tRNA. In fact, we found the circularized intron in methanogenic archaeon, Methanosarcina acetivorans, cells. Whereas the circularized intron has any function or not, we believe it would be functional because of the following facts. First, the circularized intron was stable in M. acetivorans cells. Second, RtcB even exists in bacterial cells that any tRNA gene does not contain intron. In future, we would like to discover the functions of small circularized RNAs.
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Research Products
(6 results)
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[Journal Article] Protein fishing using magnetic nanobeads containingcal modulinsite - specifically immobilized via an azido group2013
Author(s)
Ikeda-Boku, A., Kondo, K., Ohno, S., Yoshida, E., Yokogawa, T., Hayashi, N. and Nishikawa, K.
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Journal Title
J. Biochem.
Volume: 154
Pages: 159-65
DOI
Peer Reviewed
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[Journal Article] A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli2013
Author(s)
Ikeda-Boku, A., Ohno, S.,. Hibino, Y., Yokogawa, T., Hayashi, N. and Nishikawa, K.
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Journal Title
J. Biochem.
Volume: 153
Pages: 317-326
DOI
Peer Reviewed
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[Journal Article] Purification and comparison of native and recombinant tRNA-guanine transglycosylases from Methanosarcina acetivorans2013
Author(s)
Nomura, Y., Onda, Y., Ohno, S., Taniguchi, H., Ando, K., Oka, N., Nishikawa, K. and Yokogawa, T.
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Journal Title
Protein Expr. Purif.
Volume: 88
Pages: 13-19
DOI
Peer Reviewed
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