Inspection of mechanism for recognition of host by phage

2014 Fiscal Year Final Research Report

• Project/Area Number: 23570211
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Osaka University
• Principal Investigator: YONESAKI Tetsuro 大阪大学, 理学(系)研究科(研究院), 教授 (90115965)
• Co-Investigator(Kenkyū-buntansha): OTSUKA Yuichi 大阪大学, 大学院理学研究科, 助教 (10548861)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Keywords: T４ファージ / 大腸菌 / OmpC / LPS / 吸着 / 尾部繊維先端部位

Outline of Final Research Achievements

T4 adsorption to E. coli K12 strain depends on the presence of OmpC, but T4 can adsorb to B strain independently of OmpC. Using K12 mutants defective in various genes required for LPS synthesis, we systematically analyzed the adsorption of T4 phage to these mutants dependent on or independent of OmpC. Furthermore, we isolated T4 mutants which can adsorb to B strain but not to K12 strain or vice versa. These results strongly suggest that T4 has two different mechanisms for adsorption to host cells. In addition, present study identified specific structures in OmpC and the distal tip of T4 long tail fiber, required for the recognition of OmpC by T4.

Free Research Field

分子生物学