Regulation of nuclear receptors by SUMO through recruitment of chromatin remodeling factors

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570219
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: OGAWA Hidesato 独立行政法人情報通信研究機構, 未来ICT研究所 バイオICT研究室, 主任研究員 (20370132)
• Co-Investigator(Renkei-kenkyūsha): MOROHASHI Ken-ichirou 九州大学, 医学研究院, 教授 (30183114) ; TAKEUCHI Jun 東京大学, 分子細胞生物学研究所, 准教授 (10451999)
• Project Period (FY): 2011 – 2013
• Keywords: 転写 / クロマチン / 核内受容体

Research Abstract

For understanding on the molecular processes of development, cell growth and homeostasis, it is required to reveal a regulation mechanism of binding of the transcription factors to the target genes. One of a useful method to introduce a new insight into the complex formation with the target DNA is to visualize the processes using the cell line that possesses a tandem repeat array of the mouse mammary tumor virus (MMTV) promoter that is the target of the steroid receptor (GR, glucocorticoid receptor). Using this system, we succeeded in finding of a novel complex formation on the MMTV array. Although the chromatin-remodeling factor, ARIP4, is localized on nuclear structure without the glucocorticoid, after addition of the ligand, ARIP4 is co-localized with GR on the target DNA. This suggests that the MMTV array system is useful to analyze an important role of nuclear structure in the regulation of nuclear transcriptional complex formation.

Research Products

• [Journal Article] Glycolytic genes as the targets of a nuclear receptor Ad4BP/SF-1 (2014)
  • Author(s): Baba T, Otake H, Sato T, Miyabayashi K, Shishido Y, Wang C, Shima Y, Kimura H, Yagi M, Ishihara Y, Hino S, Ogawa H, Nakao M, Yamazaki T, Kang D, Ohkawa Y, Suyama M, Chung BC, and Morohashi K.
  • Journal Title: Nature Communication Volume: 5 Pages: 3634
  • Peer Reviewed
• [Journal Article] Aristaless Related Homeobox Gene, Arx, Is Implicated in Mouse Fetal Leydig Cell Differentiation Possibly through Expressing in the Progenitor Cells (2013)
  • Author(s): Miyabayashi K, Katoh-Fukui Y, Ogawa H, Baba T, Shima Y, Sugiyama N, Kitamura K, and Morohashi K.
  • Journal Title: PLoS ONE. Volume: 8(6) Pages: 68050
  • Peer Reviewed
• [Journal Article] Exportin 4 interacts with Sox9 through the HMG box and inhibits the DNA binding of Sox9 (2011)
  • Author(s): Tsuchiya M, Ogawa H, Suzuki T, Sugiyama N, Haraguchi T and Hiraoka Y.
  • Journal Title: PLoS ONE. Volume: 6(10) Pages: 25694
  • Peer Reviewed
• [Presentation] 性差による心発生異常と心疾患 (2013)
  • Author(s): 浅野哲也, 小川英知, 森下環, 小島瑞代, 土屋恵, 福井由宇子, 原口徳子, 平岡泰, 諸橋憲一郎, 小柴竹内和子, 竹内純
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド, 兵庫県
  • Year and Date: 2013-12-09
• [Presentation] SUMO化依存的クロマチン構造変換因子ARIP4の核内受容体との複合体形成および核内動態の解析 (2013)
  • Author(s): 小川英知, 土屋惠, 原口徳子, 平岡泰
  • Organizer: 第65回日本細胞生物学会大会ウインクあいち
  • Place of Presentation: 愛知県
  • Year and Date: 2013-06-20
• [Presentation] クロマチン構造変換因子ARIP4の核内受容体との複合体形成および核内動態の解析 (2012)
  • Author(s): 小川英知, 土屋惠, 原口徳子, 平岡泰
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡, 福岡県
  • Year and Date: 2012-12-13
• [Presentation] Exportin 4 interacts with Sox9 through the HMG box and inhibits the DNA binding of Sox9 JSCB2012 (2012)
  • Author(s): 小川英知, 土屋惠, 原口徳子, 平岡泰
  • Organizer: 第45回発生生物学会・第64回細胞生物学会合同大会
  • Place of Presentation: 神戸国際会議場・神戸商工会議所, 兵庫県
  • Year and Date: 2012-05-31
• [Presentation] HMG box転写因子Sox9のゲノムDNAへの結合調節機構Exportin 4 interacts with Sox9 through the HMG box and inhibits the DNA binding of Sox (2011)
  • Author(s): 土屋惠 小川英知,原口徳子,平岡泰
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜, 神奈川県
  • Year and Date: 2011-12-16