Epiblast pluripotency regulated by Nodal signal

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570233
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: SUMI tomoyuki 九州大学, 医学(系)研究科(研究院), 研究員 (90378894)
• Project Period (FY): 2011 – 2013
• Keywords: 幹細胞 / エピブラスト / Wntシグナル

Research Abstract

Epiblast stem cells (EpiSCs) are primed pluripotent stem cells and can be derived from postimplantation mouse embryos. We now show that the absence of canonical Wnt/beta-catenin signaling is essential for maintenance of the undifferentiated state in mouse EpiSCs and in the epiblast of mouse embryos. Attenuation of Wnt signaling with the small-molecule inhibitor XAV939 or deletion of the beta-catenin gene blocked spontaneous differentiation of EpiSCs toward mesoderm and enhanced the expression of pluripotency factor genes, allowing propagation of EpiSCs as a homogeneous population. EpiSCs were efficiently established and propagated in the presence of both XAV939. Such an improvement in the homogeneity of pluripotency achieved with the use of a Wnt inhibitor should prove advantageous for manipulation of primed pluripotent stem cells.

Research Products

• [Journal Article] Wnt signaling regulates left-right axis formation in the node of mouse embryos (2013)
  • Author(s): Keiko Kitajima, Shinya Oki, Yasuyuki Ohkawa, Tomoyuki Sumi, Chikara Meno.
  • Journal Title: Developmental biology Volume: 380 Pages: 222-232
  • DOI: 10.1016/j.ydbio.2013.05.011
  • Peer Reviewed
• [Journal Article] Epiblast ground state is controlled by canonical Wnt/β-catenin signaling in the postimplantation mouse embryo and epiblast stem cells (2013)
  • Author(s): Tomoyuki Sumi, Shinya Oki, Keiko Kitajima, Chikara Meno.
  • Journal Title: Plos One Volume: 8 Pages: e63378
  • DOI: 10.1371/journal.pone.0063378
  • Peer Reviewed
• [Journal Article] The SMAD2/3 corepressor SNON maintains pluripotency through selective repression of mesendodermal genes in human ES cells (2012)
  • Author(s): Norihiro Tsuneyoshi, Ee Kim Tan, Akila Sadasivam, Yogavalli Poobalan, Tomoyuki Sumi, Norio Nakatsuji, Hirofumi Suemori and N. Ray Dunn.
  • Journal Title: Genes & Development Volume: 26 Pages: 2471-2476
  • DOI: 10.1101/gad.201772.112
  • Peer Reviewed
• [Journal Article] Simple and highly efficient method for production of endothelial cells from human embryonic stem cells (2011)
  • Author(s): Rie Tatsumi, Yutaka Suzuki, Tomoyuki Sumi, Masakatsu Sone , Hirofumi Suemori, Norio Nakatsuji.
  • Journal Title: Cell Transplantation Volume: 20 Pages: 1423-1430
  • DOI: 10.3727/096368910X547444
  • Peer Reviewed
• [Journal Article] Neurexin-1, a presynaptic adhesion molecule, localizes at the slit diaphragm of the glomerular podocytes in kidneys (2011)
  • Author(s): Akira Saito, Naoko Miyauchi, Takeko Hashimoto, Tamaki Karasawa, Gi Dong Han, Mutsumi Kayaba, Tomoyuki Sumi, Masayuki Tomita, Yohei Ikezumi, Kenji Suzuki, Yasushi Koitabashi, Fujio Shimizu, and Hiroshi Kawachi.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol. Volume: 300 Pages: R340-R348
  • DOI: 10.1152/ajpregu.00640.2009
  • Peer Reviewed
• [Remarks]
  • URL: http://www.dev.med.kyushu-u.ac.jp/