2013 Fiscal Year Final Research Report
Regulation of HIPK2 and TCF proteins by Wnt signaling
| Project/Area Number |
23570235
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Cell biology
|
|---|
| Research Institution | Kyushu University |
|---|
Principal Investigator |
HIKASA Hiroki 九州大学, 生体防御医学研究所, 助教 (40596839)
|
|---|
| Project Period (FY) |
2011 – 2013
|
| Keywords | Wnt / HIPK2 / TCF proteins / Switch mechanism |
|---|
| Research Abstract |
Previously we demonstrated that TCF3 phosphorylation by Wnt/HIPK2 signaling is essential for embryonic axis specification. However, there had been still a missing link between Wnt ligands and HIPK2, which may be able to explain how Wnt stimulation trigger HIPK2-mediated phosphorylation of TCF3. In this study, we reported that GSK3 inhibition triggers the phosphorylation of TCF3, a repressor type of TCF proteins and that phosphorylated TCF3 is replaced on the target promoter with a positively acting TCF1 upon Wnt/HIPK2-mediated phosphorylation. Moreover, we reviewed recent findings referring a role of Wnt signaling during vertebrate axis specification in a journal and a book. We are currently working on regulation of HIPK2 by GSK3 and a mutual interaction between HIPK2/TCF3 complex and a novel HIPK2-interacting protein identified by expression screen.
|
