Regulation of plasmalogen synthesis and establishment of plasmalogen diversity

2013 Fiscal Year Final Research Report

• Project/Area Number: 23570236
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Kyushu University
• Principal Investigator: HONSHO Masanori 九州大学, 理学(系)研究科(研究院), 特任准教授 (90372747)
• Project Period (FY): 2011 – 2013
• Keywords: プラスマローゲン / エーテルリン脂質 / 生体膜 / ペルオキシソーム

Research Abstract

The aim of this study is to investigate molecular mechanism of the regulation of plasmalogen synthesis. Plasmalogen is a subtype of glycerophospholipids, in which a long chain fatty alcohol is linked at sn-1 position through a vinyl ether bond. Peroxisomal fatty acyl-CoA reductase 1 (Far1) is essential for supplying fatty alcohols required for ether bond formation in plasmalogen synthesis. Plasmalogen synthesis is regulated by modulating Far1 stability in a plasmalogen-dependent manner.
Here I found that Far1 is a peroxisomal tail-anchored protein. Far1, but not Far2, was preferentially degraded in response to the cellular level of plasmalogens. The region flanking the transmembrane domain of Far1 is required for plasmalogen-dependent degradation of Far1. The diversity of plasmalogens was formed through remodeling of fatty acids and Far1 stability is regulated in a manner dependent on certain type of plasmalogens.

Research Products

• [Journal Article] Molecular complex coordinating peroxisome morphogenesis. In : Brocard, C. and Hartig, A. (eds) (2014)
  • Author(s): Fujiki, Y., Itoyama, A., Abe, Y., and Honsho, M.
  • Journal Title: Molecular Machines Involved in Peroxisomes Maintenance Berlin, Springer-Verlag Volume: (in press)
• [Journal Article] Very-long-chain polyunsaturated fatty acids accumulate in phosphatidylcholine of fibroblasts from patients with Zellweger syndrome and acyl-CoA oxidase1 deficiency (2014)
  • Author(s): Abe, Y., Honsho, M., Nakanishi, H., Taguchi, R., and Fujiki, Y.
  • Journal Title: Biochim Biophys Acta Mol. Cell Biol. Lipids. Volume: 1841 Pages: 610-619
  • DOI: 10.1016/j.bbalip.2014.01.001
• [Journal Article] Topogenesis and Homeostasis of Fatty Acyl-CoA Reductase 1 (2013)
  • Author(s): Honsho, M., Asaoku, S., Fukumoto, K., and Fujiki, Y.
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 34588-34598
  • DOI: 10.1074/jbc.M113.498345
• [Journal Article] Mff functions with Pex11p ß and DLP1 in peroxisomal fission (2013)
  • Author(s): Itoyama, A., Michiyuki, S., Honsho, M., Yamamoto, T., Moser, A., Yoshida, Y., and Fujiki, Y.
  • Journal Title: Biol. Open. Volume: 14 Pages: 998-1006
  • DOI: 10.1242/bio.20135298
• [Journal Article] Docosahexaenoic acid mediates peroxisomal elongation, a prerequisite for peroxisome division (2012)
  • Author(s): Itoyama, A., Honsho, M., Abe, Y., Moser, A., Yoshida, Y., and Fujiki, Y.
  • Journal Title: J. Cell Sci. Volume: 125 Pages: 589-602
  • DOI: 10.1242/jcs.087452
• [Presentation] エーテルリン脂質プラスマローゲンによるコレステロール生合成の制御 (2013)
  • Author(s): 本庄雅則
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフイコ横浜
  • Year and Date: 2013-09-13
• [Presentation] 細胞内プラスマローゲン量によるコレステロール生合成調節 (2012)
  • Author(s): 本庄雅則
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-15
• [Presentation] 細胞内プラスマローゲン量によるコレステロール生合成調節 (2012)
  • Author(s): 本庄雅則
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 九州大学医学部百年講堂(福岡市)
  • Year and Date: 2012-06-07
• [Presentation] Fatty Acyl-CoA reductase 1, a peroxisomal C-tail anchored protein, controls plasmalogen biosynthesis (2011)
  • Author(s): 本庄雅則
  • Organizer: International Symposium on New Aspects of Phospholipid Biology and Medicine
  • Place of Presentation: ホテルルイガンス(福岡市)
  • Year and Date: 2011-11-15
• [Presentation] エーテルリン脂質プラスマローゲンの生合成を制御するFatty acyl-CoA reductase 1の活性制御にはペルオキシソーム膜タンパク質が関与する (2011)
  • Author(s): 本庄雅則
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Book] 医学のあゆみ (2014)
  • Author(s): 藤木幸夫, 本庄雅則
  • Total Pages: 248(1143-1149)
  • Publisher: 医歯薬出版株式会社
• [Book] ペルオキシソームの形成・制御とその障害 (2012)
  • Author(s): 藤木幸夫, 宮田暖, 奥本寛治, 田村茂彦, 糸山彰徳, 本庄雅則
  • Total Pages: 63(448-451)
  • Publisher: 医学書院
• [Remarks]
  • URL: http://133.5.26.69